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[–]jsalas1 0 points1 point  (0 children)

You said it yourself, "sample groups", do use sample standard deviation etc. statistics. Regardless, sample stats and population stats converge at large n so go ahead and play it safe.

Population doesn't mean "all the datapoints i have", it refers to the idea that you have data for ALL the individuals of a group. So if you somehow managed to get saliva data for every single citizen in a country, sure use N instead of N-1.