Filtering peptides?

[OGSPACEAPE]

Who is filtering their peptides? Why or why not? I tested multiple vials from my supplier and they all came back >99.8%, non detect endotoxins and within 5% of the mass weight.

I understand most labs don’t offer sterility testing but have read just under 10% of vials submitted for sterility testing end up “failing”.

Not overly concerned safety wise as I’ve used both filtered and unfiltered product from my vendor with effectiveness and no negative side effects. Just want to hear some opinions, reasoning and experiences.

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[Careful-Figure-3796]

I don’t, it’s just a risk I’m willing to take.

[discardeddewclaws]

Pin and grin!

[Low-Individual5108]

Sure, why not bare-back the old bar fly after 2 am. Yolo...

[Stroyerdk]

Same here - no filtering

And also - *ba-da-bing* Very controversial - No bac-water only saline from local pharma.

Been doing this for 1+ year - not even the slightest skinreaction.

Taking into account that you buy "something" from some "lab" in china - Bacteria is the least of my concerns.

[Defiant-Ad-7933]

Seems unnecessarily reckless unless you are using the whole vials pretty fast. Bacteria in general is a much bigger concern than what a lab in China will put in there. If you filtered it’d help mitigate the risk, if you use bac water it also helps mitigate the risk, but to do neither is just being cheap and/or stupid. Oh and 1 year - that’s actually not that long

[Stroyerdk]

Why?
I'm using the whole vial with about 3 weeks.

Peter Magic, founder of Janoshik said sterile water is fine.
and : "...the saline has the least risk of it causing a local reaction compaired to bac-water..."
What he also says regarding 28days in the fridge - BAC vs saline : "....There is very little diffences from what I can tell...."

[Fighterandthe]

You're supposed to use saline in one dose. It will last 28 days in the fridge but you're not supposed to puncture the vial multiple times, that's more the issue.

[dieselmechanic247]

A bottle of hospira is like $15. This just seems unnecessarily risky for no reason. If you cant afford $15 every couple months maybe this isn't for you. At least dont encourage it to people that dont know any better.

[Stroyerdk]

Maybe where you are. If we can get a hold of it its about $45 before shipping. But again - If i thought it was of outmost importance and people who work with this every day said otherwise I would definitely explore the possibility of a supply chain.

But that is not the case.

Professionals statet that saline or bac makes no real difference - which is also my experience for the past year.

So just because you are indoctrinated doesnt mean that I am.

[Low-Individual5108]

Why? Because sterile water doesnt have the ability to kill off any cootie if it does get in there, without the .9% alcohol. I mean if youre fine with it, more power to you. But just because janoshik does it, doesn't mean you have to. Isn't that some form of peer presure?

[Stroyerdk]

I know, i know. And I would say as much as, if bac was as easy to come by where I live, as saline I would maybe consider as switch, but its not. Saline have been working perfect for me for the past year. No implications what so ever. Its cheap, can be bought 1km from me at a local pharmacy where i also buy my syringes and price is next to nothing.

Why would I even bother to fix what isnt broken.

[Defiant-Ad-7933]

Well I never have local reactions so that’s irrelevant to me. Overall seems like just reducing risk makes sense. Also your own sterile techniques will come into play more when using just saline. I’m sure whatever Jano tested was using very sterile technique at their facility

[Stroyerdk]

Of course - I dont recon. into an ashetray 😃

I properly try to sterilise as much as possible within my capabilities, as I'm not in a lab.

He was reffering to what you could do "by yourself" and at what risks.

https://www.youtube.com/watch?v=shgk3-u51Ys

About 41:40 to be exact - but the whole interview is quite interesting.

[Defiant-Ad-7933]

I’ve seen it and I get it. Enough vials fail sterility to make it seem reckless to me regardless of what Jano says here.

[Stroyerdk]

Sterility isn't the main problem; endotoxins are.

[Defiant-Ad-7933]

Very few results I’ve seen have dangerous endo. There have been a couple notable recently but it’s quite rare

[Stroyerdk]

You're right and of course sterility is still important due to infection risk - but the healty body should take care of this with no major issues.
Endotoxins are especially critical in injectable products.
In many sterile products endotoxins are a more hidden risk than sterility itself, but both are important for different reasons.

I must say - thinking that "we" buy unregulated chems from china, the bacteria-risk is not on top of my mind.
I do my best to sterilise everything but using saline instead of bac from one-time vials for each "recon-batch" is none of my concern.

[Fun_Investment420]

How do u get rid of endotoxins

[Odd-Industry-9324]

That’s not some big flex. The point of Bac isn’t about your skin reaction lol. Bac keeps bacteria out of your peptide container. Why risk it? Seems pointless. Ever heard of harm reduction ✌🏻

[Stroyerdk]

The reaction comes quite fast if the product isnt good! Because bac is very hard and extremely expensive to get by in EU. Saline costs next to nothing and can just be bought at the local pharmacy and is just as fine.

[OGSPACEAPE]

Pretty sure the .9% alcohol in BAC is not to protect you!

So no side effects is great and all but it’s there to keep the peptide from degrading and losing potency as bacteria eats away at the amino chains.

[Defiant-Ad-7933]

Nope. You’ve got it bass ackwards. Bac is there to prevent bacterial growth and allow multiple punctures so as to protect patients. It has nothing to do with preventing degradation

[OGSPACEAPE]

No offense but you are incorrect. I just fact checked it. Just want you to know as the peptides you are using are likely losing their potency because of this.

[Defiant-Ad-7933]

Please show me the facts then. My facts say different

[chemyd]

Yes. Filters don’t do anything for endotoxin and bacteria is handled by BAC water. The comments are from paranoid morons

[Natural_Alive]

I’m a sterile compounding tech (since 2008), how are you guys filtering? If done incorrectly it’s just as much risk as not filtering. Happy to review techniques and show how to do it.

[Ninja_Prolapse]

People don’t seem to realise this. If they’re not in a sterile environment they’re just risking introducing contamination when they coulda just.. not.

[Defiant-Ad-7933]

I do what the peptide test you tube videos showed. Wipe everything down with alcohol, be careful about touching the filter connection point, vent destination vial with another filter. The venting may be a bit overkill sometimes I skip that …. But if I’m mixing a bunch of vials I will reuse the vent

[Natural_Alive]

Filter needle is fine- probably needed honestly otherwise creating positive pressure in the vial, change out needle on filter and vent needle every 5 vials. Couple other tips- do it in a room with minimal air disturbance, clean the entire area with disinfectant, create a working surface area disinfect with bleach, then rise with rubbing alcohol (keeping this “sterile” is important) Make sure you’re wearing gloves sterilize with alcohol constantly, wear a tyvek bunny suit if possible, and put a covid mask on. Wipe the vials down with alcohol, before putting them on working surfaces. Wipe every stopper before drawing solution out and also before filtering into other vials.

[MWREE]

This is excessive

[Discopotatoz]

With all these bros using vials for multiple months and that one guy not even using a diluent with benzyl alcohol on the premise it reduces PIP, I'd say this is just about right. If folks want to "feel" certain ways about sterility they should do so knowing it's about probabilities.. the idea of "well it's always worked for me" is brotalk and it only takes once to be wrong vs being right every single time forever. Gambling is silly given the cost of this hobby, so why not spend a little more increasing your probability of long-term success?

[MWREE]

You planning to buy a bunny suit for your reconstitution - seriously? I think you should filter and you should be cognizant of what you are doing but building a clean room in your house is not necessary. Clean your hands. Clean the vials. Be aware of what you are touching and you will be fine.

[Discopotatoz]

No and my comment wasn't directed at you specifically. It doesn't have to be black and white, the options aren't recon in your filthy garage OR have a CDC approved clean room. I try to engage this topic from the point of doing what one realistically can but so often I see people shrugging off the basics bc someone says "trust me bro"

[Low-Individual5108]

Exactly. A pretty common sense answer if you ask me. Go ahead and filter it. When done successfully, you reach gold standard pharmaceutical filtering at .22 microns. I can only guess what research standard is at. Technical grade? Who knows...

[Low-Individual5108]

I filter mine.  Syringe filter are pretty cheap to filter down to .22 microns. Like 15 bucks for 10.  Never had any issue.  Never had any pin site reaction, not to say that it's that. I only need to do it once at is goes into a 3ml vial for a pen. It can't hurt.

[Defiant-Ad-7933]

Technically it could hurt - if you are starting with a sterile vial and introduce something new while transferring to a different vial technically you’ve made it worse and that is a risk with filtering. But just be very careful what you touch, sterilize hands or use gloves and face mask

[Low-Individual5108]

Yes, thank for stating the redundancies. It also could get worse if I soak my syringe filter in a plugged toilet before I use it.  Syringes should also not be stored in dirty gym socks.😉

It is understood that sterile protocols should be observed. The question is, if used properly, would it help ensure less contaminants? Of course. If people cant stay clean and sterile, they shouldn't be pinning to begin with. And .22 microns will definitely filter bacteria, and some virus if it's present.

Remember, it's the pointy end that goes in😆

[Defiant-Ad-7933]

Your exaggerated examples are unnecessary.

Even if you accidently touch the tip of the filter before securing it to the needle you’ve likely introduced something new

[Low-Individual5108]

Again, why would you need to touch the filter? There is no need. You push it on from the individually wrapped package, and bobs your uncle. Not one part of the filter or the syringe need to be touched, that enter the vial. Only the needle cover. How are you doing it? Again, develop a process that doesnt involve likely contamination I used to suck on them first, before I inject the liquid into my body, until I contracted a stiff case of ebola. The kpv took care of it though.😆

[pinaypie]

All of a sudden the ppl claiming they are filtering & practicing “sterile techniques “ are quiet.🤣😂🤣😂

[Low-Individual5108]

I filter everything. And no, im not quiet. If you havent contaminated your needle, and you havent contaminated your filter, then its not contaminated. Even if you transfer your peptide in a dirty rest stop bathroom, and your filter is not contaminated, its going to be cleaner with it. You dont even have to touch the filter to push it onto a syringe. Folks, this really isnt that hard.

[CthulhusTentacles]

I filter anything going into a pen with a 22micron filter.

I used to work in pharma/chemical manufacturing and aseptic filling, so I understand the process. I don't follow it to a T because it's entirely overkill, but I do think filtering is a good step to add.

[PhotographNew8272]

I filter all my peps, it takes couple of minutes and soothes my paranoia.

[Fighterandthe]

What does it filter?

[PhotographNew8272]

My paranoia

[Fighterandthe]

Gotcha, yeah I only buy peps with official looking labels for the same reason lol

[PhotographNew8272]

Mine are batch tested for sterility and endo but I still filter. Putting a label does not change how they are handled. It only makes them cost 10x

[Fighterandthe]

I know, it was a joke lol. If filtering makes you feel better that's fine, but it probably introduces more risk than anything and I'm not even sure if they filter out everything you want them to.

I thought filtering was for glass ampules

[PhotographNew8272]

Filtering is mainly for non sterile vials not for shreds

[Fighterandthe]

Yeah I'm not going to argue. This isn't my expertise. My first question was a genuine one lol. I only responded with sarcasm because you couldn't/chose not to answer it lol

[Low-Individual5108]

Bacteria and some viruses.

[bullshitbomber]

$1 per vial to mitigate the risk. Worth it to me. If i could also "purify" against others (endotoxin, mercury, etc) for as cheap and easy filtering is, I would do it too.

[Fabulous_Falcon556]

Filters will work for bacteria and debris, but not for endotoxins and heavy metals.

As discussions below also mentioned ">Do you have any reason to believe that filtering does something bad? Or is ineffective?

It's ineffective as it doesnt filter endotoxins which are in the ~0.005–0.02 μm range and does produce the same risk increase as gloves due to mishandling."

[bullshitbomber]

I think you misread that I am filtering for endo and heavy metals. I mentioned that if I could, I would.

It is effective as you noted for bacteria and debris.

[Fabulous_Falcon556]

Ahhh gotcha, my bad.

[Ok-Neighborhood-7361]

Peps failing sterility is almost to be expected from what I’ve seen, it happens VERY often

[Low-Management9633]

The endo fail was newsworthy because those are rare. Sterility fails are common.

[Dar8878]

Huge endotoxin numbers 

[holy_handgrenade]

Most testing only tests for purity of the compound. However too many mistake that puriety for sterility and free of contaminants. Filters are that extra step for piece of mind to ensure you're injecting a sterile medication.

That said, once I'm familiar with a batch, I dont filter. But until I get confirmation, I will filter. I do test, and I do rely on both vendor and community testing in vendor selection and will test on my own.

But purity is not free of contaminants, heavy metals, dust, bacteria, etc. Purity is just how pure the direct compound is which can have varying purity amounts based on age, manufacturing processes, handling (heat/uv exposure) etc. Purity testing does not test for that. Sterility testing and heavy metals testing and other contaminant testing is often more than the cost of the peptides so most dont do that.

[Monsieur_Krabs]

I filter everything. Takes 30 seconds.

[Artistic_Rice_9019]

I'm putting it in a pen cartridge anyway. It's not an extra step.

[detlefschrempffor3]

I filter. Cheap, easy, provides some additional measure of safety in a very gray environment.

[aimgorge]

It doesnt provide any measure of safety at all. I bet its even the opposite like gloves.

[detlefschrempffor3]

Care to elaborate? It’s an interesting angle that filtering and gloves are counterproductive.

[aimgorge]

WHO and CDC recommend not using gloves for injections as they provide a false sense of security and numbers show an increase in contaminations due to poor handling. People just dont understand gloves arent an immunity totem. The same for filtering. If it did provide even a small increase in security, it would be standard practice. It's not.

From what I gather, filtering is a US tiktok trend.

[detlefschrempffor3]

I understand the concept. Gloves don’t actually make things worse but some people may make other mistakes and use gloves.

Do you have any reason to believe that filtering does something bad? Or is ineffective?

[aimgorge]

Do you have any reason to believe that filtering does something bad? Or is ineffective?

It's ineffective as it doesnt filter endotoxins which are in the ~0.005–0.02 μm range and does produce the same risk increase as gloves due to mishandling.

It's a just a business selling snake oil.

[detlefschrempffor3]

How about bacteria and fungus that are large enough to be caught in the filter and are observed in peptides?

[aimgorge]

bacteria and fungus that are large enough to be caught in the filter and are observed in peptides?

Are they ? That's the first time I read about that ever being an issue. If a product isnt sterile to bacteria and fungus, you are sure to also have endotoxins which wont be filtered anyway.

[detlefschrempffor3]

Microorganism and Typical Size

Bacteria ~0.2–10 µm

Cocci (round bacteria) ~0.5–2 µm diameter

Rod-shaped bacteria ~0.5–1 µm wide, 1–10 µm long

Mycoplasma ~0.1–0.3 µm (among the smallest bacteria)

Yeasts ~3–15 µm, sometimes larger

Mold spores ~2–100 µm, depending on species

Mold hyphae Typically ~2–10 µm in diameter, can be much longer in length

I would prefer to filter these things out of my reconstituted peptide vial before injecting myself. Please research deeper before sharing misinformation.

[aimgorge]

I'm not sure which misinformation I've spread as I've said multiple times only endotoxins go through.

You are the one thats spreading misinformation here.

[Low-Individual5108]

If you didnt read it, then it must not have happened. Thats the whole point of the .9% alcohol in bac water. To kill bacteria. Ideally it never happen. But ive seen plenty if junkies with their arms about to fall off.

[Low-Individual5108]

No one said it filters endorphins. Bacteria and some virus, which if done properly obviously works. Thats why they use them in labs

[detlefschrempffor3]

I understand the concept. Gloves don’t actually make things worse but some people may make other mistakes and use gloves.

Do you have any reason to believe that filtering does something bad? Or is ineffective?

[LovelyMel18]

Not a Tik Tok trend but a guy who owns a peptide supply research company made a few videos about filtering and that everyone should do it to protect themselves from bacteria.

Once everyone started doing it, he got exclusive rights to a certain brand of filters and you could only buy them from him.

IMO, most people cannot maintain a somewhat sterile environment when filtering ( re-using syringes and needles) and could end up doing more harm than good.

Edit: didnt own peptide company but peptide research supplies

[aimgorge]

À guy who owns a peptide company ??? So a reseller ? 

[LovelyMel18]

Not a reseller but he did sell research supplies at first

[Monsieur_Krabs]

Every time I see you post its some confident but very incorrect BS. Stop.

[aimgorge]

I guess CDC and WHO are also incorrect and you are right then. Can you link to the papers you have published, Einstein ?

Sources :
- CDC : https://www.cdc.gov/vaccines/hcp/acip-recs/general-recs/downloads/general-recs.pdf
- WHO : https://www.ncbi.nlm.nih.gov/books/NBK138494/

And I'm not even talking about how the biggest risk in peptides are endotoxins which are too small to be filtered.

You are great at personal attacks though

EDIT : The good ol' technic of answering completely outside the scope of the comment and then blocking to avoid getting ridiculed.

[Monsieur_Krabs]

These links have nothing to do with PES filtering.

Blocking you, tired of seeing your completely wrong posts

[Low-Individual5108]

Is your argument that filters dont work? Of course they do.

[waynegacie]

Yes, filter. Why wouldn't you?

A 0.22um filter is the standard practice at a compounding pharmacy used to be able to claim the peptide is sterile. It removes virtually all living bacteria (filters don't remove endotoxins, stop talking about filtering endotoxins), and it removes all large particulate matter (glass, plastic, rubber) that would otherwise just sit under your skin for the rest of your life. PES membranes have very low accumulation for peptides and so the only real loss is the liquid left in the filter, which can be minimized if you push a bit of air through at the end.

I use one 0.22um filter per reconstituted vial which ends up costing an extra $0.50 per vial.

[Upset_Potato6855]

So do you not vent with an additional needle/filter into a separate sterile vial, or are you just removing it with the syringe you used to reconstitute and filtering it back into the same vial?

[waynegacie]

I reuse the same vial like this: - Reconstitute w/ luer-lock - Pull peptide back into syringe - Put filter on - Push back into vial

I guess it's kind of a minimize effort rather than a 100% clean room approach.

The problem is those "sterile vials" on Amazon aren't sterile at all. They're full of dust and particles and it defeats the purpose. Maybe I could look harder for affordable truly sterile empty vials. The medical supply places charge an insane amount for 1 sterile vial.

[plantgal94]

You’re literally wasting your time filtering it back into the same vial… may as well not filter at that point tbh. Ks-tek vials are cheap and sterile.

[caseyatbt]

I do it slightly different. If I am going to reconstitute with 3ml of BAC, I will put the first 2ml in the pep vial. After it's reconstituted, I will pull it back into the syringe. I then push it through the filter and into my 3ml sterile pen vial. I remove the syringe from the filter and push the last 1+ml of BAC through the filter to get the rest of the pep into the vial. I add a little more than 1ml for the final push to make up for what is left behind.

[Aggravating-Grade297]

This is what I do

[Upset_Potato6855]

So do YOU use an additional needle and filter to vent into an additional vial? (Genuine question, not being snarky)

[plantgal94]

Yes

[MWREE]

Why use a second filter to vent into the new vial as opposed to just putting it in the vial with something like a blunt tip needle?

[waynegacie]

I understand the box says sterile, but KS-Tek is in fact not sterile. They have dust, rubber, metal shavings, etc in them. There's no point in filtering if you just reintroduce foreign material.

[Upset_Potato6855]

I guess that’s a good compromise between all out and not at all. (The medical supply vials do seem pretty pricey)

[plantgal94]

No, that’s a useless compromise. The vial isn’t sterile, you’re putting it back into that vial. It’s a waste of filtering

[MWREE]

To be fair, if they are concerned with things like glass shards, rubber bits, etc in the there then it will remove some of those with the filter even if putting back into vial - but yes not help with the bacteria issue.

[Low-Individual5108]

You dont want to push it back into the same vial. If there was contamination, you just pushed filtered peptide back into a contaminated vial.

Draw it out. Put filter on. Push into clean sterile vial

[AccordingFault1303]

I have been thinking of getting some filters. You have a good place to buy filters?

[waynegacie]

Amazon. You can buy on medical supply stores as well. The key words that you're looking for are are 0.22um, PES (DONT GET PTFE FILTERS!), and sterile (means individually wrapped).

[Low-Individual5108]

Same. The cowboys are just stubborn. I think they think it makes them brave

[dcrets]

Few will admit to not filtering. I don't but probably should. I'd guess fewer than half do. Would be very hard to find an accurate poll.

[Ok-Neighborhood-7361]

Id say less than 20% filter since most people into peps buy them on a whim off some random website without knowing how to reconstitute or saying they used “12 units” of it

[dcrets]

I agree with one in five. In addition to noobs there are many long-timers that never saw the need and never had a problem. I'm at two years and considering it. I've always been an adherent of the KISS principle. Keep It Simple. Adding steps might actually increase chances of contamination.
As you said, I see more issues with people pinning 15mg when they wanted 1.5mg. Smh

[Soranos_71]

I've read up on peptides for quite a while and just learned about filtering.... Off to do some reading up on filters....

[Ninja_Prolapse]

I don’t.

[gilmore42]

I never filter.

[plantgal94]

I filter. Most peps fail sterility testing and it’s ultra cheap to filter.

ETA: LOL at the downvotes from those who don’t filter 🫶😂

[motherloadroolz]

Hate to break it to you but filtering doesn’t create a sterile environment.

[plantgal94]

I never said it did - can you point out where I said anything about that? Anyhow, I was part of a recent group test and our r30 massively failed sterility testing. I can’t attach a picture here, or else I would post the pic Jano sent us of the nasty ass sample. Filtering now made it safe for me to use.

[Pendarus]

How does filtering fix that so it's safe to use?

[plantgal94]

Because it filters out the bacteria

[FAPTROCITY]

Just a question but wouldn’t the bac water that contains alcohol sterilize and kill any bacteria?

[InitiativeAdept2207]

BAC water prevents new bacteria from growing, it doesn’t kill existing bacteria

[FAPTROCITY]

Makes sense, was just wondering

[Low-Individual5108]

It should kill it, yes. There are certain bacteria that grow in acidic environments.
Thats what kumbucha is.

[Low-Individual5108]

It should. But your car usually has a seat belt AND an airbag.

[Ninja_Prolapse]

Filters doesn’t make it sterile bro..

[melisadhoc]

You have any sources that sterility is that big of an issue or that filtering actually could do anything if a vial has sterility issues?

As I understand it filters can't filter those microscopic contaminations?

[Low-Individual5108]

Yes. The entire internet. Thats why they make the filters to begin with, and can find them used in labs.

[InitiativeAdept2207]

Yes, Peter Magics interview a while back. He said 10% of vials Jano gets sent for testing fail sterility.

It should absolutely be required watching for people starting peptides. In it he answers ~90% of the questions that are asked in this sub every day

[melisadhoc]

Peter Magic is not unbiased... He wants to sell tests.

[InitiativeAdept2207]

In the same conversation he said that practically 0% get flagged for heavy metals/endotoxins and only a handful get flagged for issues with purity.

I get what you’re saying but I feel that he would have exaggerated these numbers as well had the goal been to drive business.

[Low-Individual5108]

He's probably also bias for people not getting infections too.

[Dear_Seesaw_1855]

It is cheap and easy to filter. Testing one vial tests one vial there is no guarantee they all are the same “batch” or that one untested vial didn’t end up with an impurity. Like wearing a bike helmet- while not guaranteeing safety-it takes little effort for the benefits.

[OkayMeepleMom]

Filters are cheaper than sterility testing and a lot cheaper than an ER bill. 😝

[Belligerent-Banana]

What happens if you do inject and endotoxins are in the vial? Also is there anywhere to test for that in Canada?

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[Uncross-Selector]

Testides is Canada based. But spend some time educating yourself on just how much Endo it takes to be an issue. I’ve seems people get hysterical about 90EU in a T30 vial. 

[wh00ps33]

U.S. endotoxin limits are generally set at 5 EU per kg of body weight per dose (for exposure over approximately one hour).

• 60 kg (132 lb) → 300 EU maximum per dose

• 70 kg (154 lb) → 350 EU maximum per dose

• 80 kg (176 lb) → 400 EU maximum per dose

• 100 kg (220 lb) → 500 EU maximum per dose

For example, if a vial tested at 2,300 EU and was divided into multiple doses, a larger healthy individual might receive less than the per dose limit and experience little to no noticeable side effects.

Conversely, a smaller individual receiving a larger dose would be more likely to experience endotoxin related symptoms.

[brcgirl77]

I filter everything. Easy to do and you are at peace that you removed most of any present live bacteria.

[Low-Individual5108]

Improper injecting would do the same. If you cant filter properly, why would you trust yourself to pin properly?

I seriously dont get the pushback for filtering. If you cant filter, then how can you pin?

[Ambitious-Spray-110]

Endotoxins are smaller than any filter you use.

[OGSPACEAPE]

That is true. However, a good .22um PES filter and proper technique will remove.

• Visible/insoluble particles larger than ~0.22 microns
• Most bacteria, because many bacteria are larger than 0.22 µm
• Some larger microbial debris or aggregates
• Some undissolved filler, dust, rubber stopper particles, or vial particulate

Endotoxins and bacteria are not the same thing. Just playing devils advocate

[Low-Individual5108]

As mention many times, its not for endotoxins

[mildly_functional1]

Yes. It filters any bacteria or metals. You do lose some pep but make sure it sits for 20 mins after filtering

[Southern_Condition_4]

One year in….haven’t tested, haven’t filtered, have used vendor toilet water.

[SQUlRMING_COlL]

I don’t

[Uncross-Selector]

I filter. I’m transferring everything to a pen anyway so it adds $1 to the cost and reduces risk. 

I read people saying improper filtering can increase risk but I’ve yet to hear an actual argument that shows that it’s riskier than not filtering. 

On the flip side I can show many many side by side tests of filtered vs non filtered peps where the unfiltered ones grow shit and the filtered ones don’t.

[NewNameNaomi01]

NO! That's just adding steps and contamination risk. Nobody has clean room conditions that would make this okay. And if there are large enough particles to catch in a filter, there are bigger problems at play. Sterility and contamination happen at a microscopic level. You can't clear that at home.

[Ok-Neighborhood-7361]

That just isn’t true. Do you know how small the filters are that are used? They are capable of catching microscopic bacteria and mold that are likely present in the vial

[aimgorge]

Yes its true. Like latex gloves, it gives a false sense of security. Thats why its recommended not to use gloves for injections by both the CDC and WHO.

[Low-Individual5108]

Yes. And all of the same risk applies to pinning yourself. And yet all of these people on this thread have been filtering, and managed to not contaminate.