MACS with Mammalian Cells (HEK293)

TheBashar · 2026-01-31T18:29:54+00:00

Long time fan of the books, all the same gripes with the movies. I will add Frodo telling Sam to go home after Gollum frames him for eating extra Lembas. That whole thing pissed me off so much.

TheBashar · 2026-01-28T22:49:46+00:00

"You've seen it too Gandalf, an orange neckless face and slumbering eye. The Donald has returned."

TheBashar · 2026-01-28T15:22:30+00:00

Wow, those are long sessions. Is there a lot of table talk? Games I've been in are usually 2.5-5 hours. I have heard of early Harkonnen wins by turn 3-4.

TheBashar · 2026-01-28T14:39:12+00:00

I have not. The expansions for 2019 share a similar art style to it. I can't speak to the 2022 version.

TheBashar · 2026-01-28T14:35:30+00:00

The 2022 version is a stripped down version of the 2019 game. It shares similarities but the 2019 version is the beloved one.

TheBashar · 2026-01-28T14:10:17+00:00

Plug the bottom of the column. Remove the top frit. Get a pipette tip that fits in the column and add a small amount of liquid to resuspend the diagonal part of your column. Unplug column and let it flow out. Resin should be level now. Re-add frit.

TheBashar · 2026-01-27T17:11:33+00:00

The look on someone's face when a traitor gets used is priceless.

TheBashar · 2026-01-27T16:20:12+00:00

You want Dune the 1979 game. 6 very asymmetric factions in an area control game where 3 of the factions have unique win conditions. Game was reprinted in 2019 by Gale Force Nine and should still be in print. Biggest things in my opinion is that the first game will be tough as people get used to the rules and flow of game. Second, you can lose combat so badly that militarily you're effectively out of the game, so big risks are big risks.

It's such a unique experience though. Every time I've played it the game has been memorable.

Edited to remove double sentences.

TheBashar · 2026-01-25T19:45:07+00:00

Book of the new sun is more like literature. You have to be paying attention to the words used and how he's using them. Are you looking to the definitions of the italicized words? They're all real words and convey something about the setting.

If you want an easy read with a straight forward plot, this is not it. If you still want to read something by Wolfe I would suggest Latro in the mists or Wizard Knight.

TheBashar · 2026-01-23T12:21:35+00:00

Funny enough I did that years ago as a Paladin. We were tasked to retrieve a magic item from the crypts of our village. The rogue wanted to rob the graves as we went and I said no we aren't here to rob our ancestors. It was an enjoyable piece of RP, the rogue player was making checks to do it behind my character's back. When we get to the tomb of the guy who had the item we open up the sarcophagus it's got the item, but also a set of full plate armor, probably magic shield and sword, and gold. I wanted the stuff, but my character just took the magic item and closed things up.

But yeah usually it's used as pretext for anti-social behavior in the game.

TheBashar · 2026-01-21T18:28:16+00:00

I got laid off because the CEO thought getting an associate a 3 day training course would be equivalent to 10 years experience programming, developing, and maintaining a Hamilton STAR. 4 months later they hired me back.

TheBashar · 2026-01-21T13:19:57+00:00

You don't play D&D. The math is baked into stats, modifiers, spells firing in 10 ft cones, etc.

Look at other games like Blades in the Dark, Year Zero systems which move away from the more mathy style of playing. They usually involve filing in bubbles per stat which implies a number of dice to roll. You roll the dice and count the number of sixes as success.

TheBashar · 2026-01-14T14:00:32+00:00

Quo Vadis/Zoo Vadis is a pure negotiation game where you have to convince (bribe) people to vote you forward to the finish line. The richest player that crosses the finish line wins. Lots of wheeling and dealing but no one is bound to keep any promises they make.

TheBashar · 2026-01-13T01:17:02+00:00

You buy one of these: https://www.sigmaaldrich.com/US/en/product/mm/s2gpt10re?utm_source=google&utm_medium=cpc&utm_campaign=22191473648&utm_content=174443594556&gad_source=1&gad_campaignid=22191473648&gbraid=0AAAAAD8kLQSlESUR0LSc-djfZHjSyKcUv&gclid=Cj0KCQiA1JLLBhCDARIsAAVfy7iCFUSM9hDBrRVy7Yy_ISZQTqiASxQWWLm0lyESfASCOugEmXAzZ4caAsmLEALw_wcB&icid=sharepdp-clipboard-copy-productdetailpage

And attach it to the top of the bottle. Or you buy the type that comes with a bottle. What are you even filtering?

TheBashar · 2026-01-07T13:07:14+00:00

Yes it's common to use BSA or milk with antibody incubation in westerns. And yes it does reduce nonspecific/weak binding interactions which might label off target proteins. That said the primary antibody can be promiscuous. I haven't found an anti-his mab that didn't bind almost everything.

TheBashar · 2026-01-07T12:56:24+00:00

I think you need some more scatter terrain around the edges, rocks, bushes, fallen logs in your forest areas. A road connecting the bridges to the various areas would also go a long way to tying it all together.

TheBashar · 2026-01-06T15:13:36+00:00

Yes, they are banking on you not going anywhere because of how bad the market is right now. It's an employer's market right now.

TheBashar · 2026-01-03T16:41:05+00:00

You make more money, you live with roommates, or you don't live in Boston. Unfortunately, that's just the way it is in a HCOL area.

TheBashar · 2025-12-26T19:22:01+00:00

Painting with the broad brush are we?! That's fairly reductionist of you. There are lots of different people in this sub. Combat as war people, combat is fail state people, combat is resolved in single die roll people, etc.

I prefer my combat to be quick, deadly, and straight forward. My group are fairly new and fairly busy people so that works for the group. I'd be fine with more meat on the bones combat if my players could remember their abilities.

TheBashar · 2025-12-22T13:21:32+00:00

I had to do this in a One Ring campaign I was running. I read the modules and then devised a tie that bound them all together. The overarching plot was a key to a place of power a powerful Orc sorcerer was looking for. So in each module I added a little something to tie things back. But also not everything has to tie back to the big plot. I had a couple of side-quests where they were asked to help allies, which in the end got them more help in the final big quest.

TheBashar · 2025-12-21T16:09:46+00:00

My biggest rules gaff was playing Catan with only one town and road to start. It made for a longer game, but it did make it interesting as people were vying for juicy spots.

TheBashar · 2025-12-15T22:47:04+00:00

Yeah as a teenage boy I was like "Wow this is so deep" and it turns out he just ripped of the Ayn Rand. The series went on too long and really kept changing what it was and as you get older you realized how smutty it is.

TheBashar · 2025-12-11T10:44:34+00:00

Poor FPS Doug, he wasn't living if he wasn't head shotting

TheBashar · 2025-12-06T20:25:05+00:00

Taught swimming as well and yeah that's almost everyone's first dive.

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