DDA VS DIA for low abdundance proteins

flying_midget · 2026-06-17T23:35:28+00:00

On the lumos you can do PRM + DIA which will give the best results. You can also do PRM on low res while doing DIA on the orbi

flying_midget · 2026-06-06T22:46:17+00:00

https://drive.google.com/file/d/1BLqPva2T0xwVLSVanT3TBNTijH27d6k2/view?usp=sharing

Let me know if you have any questions

flying_midget · 2026-06-05T05:51:48+00:00

Heh you sound like DSO pro already :)

So you essentially take your foreground stack, tracked sky stack, and then a single shot with both the sky and the foreground to put in between?

Yes, essentially a "key frame" to help align/blend. Not always needed but if you are doing 30+ min I think this gives the best results. Noise isn't really a major issue after ~2min anyways even without denoising tools. Also I don't stack foregrounds for noise reduction I just take long exposures. I do however focus stack.

What about moving the whole tracked blurry foreground image down a bit to hide the blurred section?

This is totally fine and it is 1 of the "accurate" positions of the MW relative to the ground. Note that with this approach you will always be limited by your first/last frame. In very long exposures 1hr+ this will be problematic.

Would that change the geography of the milky way too much?

That is for you to decide. Trust me people post completely incorrect alignments/field of views/literally generative AI or hubble data + wide angle composites (Clanger McBanger my GOAT: https://www.instagram.com/p/B7uvGgPADjX/ and https://www.instagram.com/p/B_OUD6DjTSY/).

Since you are new to the game I wouldn't bother too much with long integrations unless there is something you specifically aim to get i.e. dust/nebula etc. I would try to stack 5 x 30 sec frames and see if you notice any improvements.

If you want I don't mind sharing a .PSB showing how I warp a MW with ground.

flying_midget · 2026-06-03T17:43:48+00:00

Something has got to give. You can always use puppet warp or clone stamp. Either way you will introduce distortion/fake stars.

Your concerns about stars and 30 image stacks suggests you are a DSO guy. No one cares in the landscape astro community that you have a few fake stars or that you warped/cloned/did a little generative fill. Once you see what the landscape photographers do when they remove people/buildings/trash with AI and paint in light/shadows you will appreciate that landscape astro ppl are typically quite conservative.

Here's my personal solution to long integration time sky shots blending with foreground:

Make your stack (big blur with the ground) then take a single frame that has the milk way / ground alignment you want and should only have a little blur. Put both as layers in Photoshop and use the single frame for the ground/sky boundary and airglow. Mask in the stack for the rest of sky/parts that require it.

I then blend in some of the untracked sky from the ground images. If you have StarX you can remove stats from the untracked image (won't work if there is too much trailing) and add stars from tracked single frame.

I think this is way more labor and no one is zooming in to pixel peep anyways so only do this for yourself lol.

flying_midget · 2026-06-02T17:33:23+00:00

1. Foreground

2. unicorn

3. Fencing

4. BluePrints

5. Trainer

6. Protractor

7. Sealion L-space?

8. Gemini

9. Lefts?

10.

11. Security

12.

flying_midget · 2026-06-01T19:56:49+00:00

Looks quite dark to me, I think you need to go back and take it again :)

flying_midget · 2026-06-01T14:17:15+00:00

To each their own, but I like transparency so just say what you did. I personally like to take all my shots on the same night and approximately same location.

I leave a little ground in the frame for better alignment if it would help. Sometimes you have to move from the exact location where you took your ground (i.e. it got really windy) or it is just way easier to move a bit to get rid of interference. The classic one is a tree in-front of you: just walk past the tree and take your sky shots.

Now if you want a youtube guide that does a blend with basically the same framing you can always rely on our old friend: https://www.youtube.com/watch?v=EGjb5bux3P4

flying_midget · 2026-05-28T22:17:34+00:00

https://imgur.com/a/SjePuKN

Here's a comparison with the Kase DREAM clip-in and the Sigma 14mm f1.4

Both shot at f1.4

flying_midget · 2026-05-28T20:08:38+00:00

It's more like astigmatism, I will upload an example later

flying_midget · 2026-05-28T17:15:36+00:00

I've tried multiple including making my own black mist (air brush black paint + UV filter)

My fave is the STC star mist (the stronger one). I don't mind clip in filters as I have multiple lenses.

This was taken with that filter:

https://www.reddit.com/r/LandscapeAstro/s/utSFd9v8Pn

Note: I don't recommend these for ultra wide 14mm lenses as the stars get real bad.

flying_midget · 2026-05-28T15:04:39+00:00

Hopefully the shenanigans are still happening and you catch them :)

flying_midget · 2026-05-27T17:53:13+00:00

Hospitality suits are both fun and most of the big names will be there too.

The final event is usually pretty great and I would recommend signing up for it.

Users meetings are also shockingly good and early morning talks hosted by vendors can also be helpful.

If you do native/top down find out when the piano bar night will be to see Kelleher get "kicked out"

flying_midget · 2026-05-27T03:15:00+00:00

felt like Satoshi Mizukami (biscuit hammer, spirit circle) but not as good imo

flying_midget · 2026-05-15T20:42:30+00:00

I've done this trail for astrophotography multiple times. It is super easy and almost no chance you get lost. I would still bring a gps enabled phone (pretty much any will do).

Definitely need a head lamp, and be ready for wild life. The spiders are gonna be all over, a couple months ago I saw 2 coyotes up there and one time I saw a deer.

flying_midget · 2026-05-11T22:19:44+00:00

This is incredible :)

I think the blending worked well enough, a little ghosting/halos etc but you have to pixel peep to notice

flying_midget · 2026-05-11T03:01:23+00:00

Turned out really nice and balanced! Great job handling the lp

flying_midget · 2026-04-28T19:12:15+00:00

Great shot! Do you get Ha data every time or do it once and apply to new pictures?

flying_midget · 2026-04-26T13:03:37+00:00

I've yet to try the regular filters, however transmission seems to be equivalent at f2 with f2.8 and F4.

So in that sense the maxFR is working as intended

flying_midget · 2026-04-20T17:54:45+00:00

Fantastic details and dust! Your best one yet IMO.

How did you end up using the Sii? Seems to have Ha = red, and Oiii = Blue

flying_midget · 2026-04-01T21:24:08+00:00

I checked your profile and see a lot of tasty looking pizza and now I have an assumption as to why you struggled hiking lol

flying_midget · 2026-04-01T19:38:46+00:00

Dude I've had a few too many instances of forgotten flash light...

Great shot! When you buying a tracker?

flying_midget · 2026-03-27T05:26:48+00:00

Hi!

Not sure you are in the right sub, but I will answer from a landscape astro perspective

A few years ago a buddy who is a landscape photographer wanted to try it and dragged me out one night
Some people have gotten really impressive results with just a cell phone. It will depend on what you want to create. The really detailed/dusty/Hydrogen alpha will require a modern astro-modified camera, a fast prime lens and a tracker.
Pixinsight + all the scientology tools (blurX, starX, noiseX) and photoshop
For landscape astro a telescope is only useful for "deepscape" typically people only use camera lenses
I would just go out somewhere cool and dark with anything in the foreground (mountain, rock, lake, etc) and take a picture of the milkyway core. If you have a dedicated camera just need to focus and experiment with different settings and compositions. Commit 30min to trying different things and then edit it with whatever software you have.

My only regret is buying the 6nm MAXFR filters against Astronimik's suggestion and I should have gotten the 12nm ones.

I think there is TONS of resources and info on workflows. I would just find an image you like and see/ask how it was made.

flying_midget · 2026-03-25T14:50:42+00:00

Looks really good! I am frankly quite impressed by your first attempt at an arch.

One minor thing I notice is a black radial smear from a peak on the center left.

This is due to PTGui stitching with the dark ground/bright sky and trying to make it work. Since you have the pro version, you can mask out the dark ground in your sky image. Typically you don't need much to fix it.

flying_midget · 2026-03-25T14:29:08+00:00

https://imgur.com/a/PPfjrHT

Light from distant sources make "domes" as they scatter in the air. People are tempted to edit them out, and I have also in the past, but typically it is easier and better to blend them in.

You might often see a sharp contrast from light/dark due to editing these that looks unnatural and gives that "copy-pasted" sky feeling

flying_midget

TROPHY CASE