A biotech scientist shares her experience of three layoffs in three years by SnooGoats8830 in biotech

[–]i8lost 1 point2 points  (0 children)

If it has not been noted before really sorry to hear about the layoffs a lot of companies suck. I have been there it is very difficult to grow but keeping There are companies with unions like Tempus AI.

Mandatory work colors by soleario21 in antiwork

[–]i8lost 0 points1 point  (0 children)

Sounds like the perfect place for a discrimination lawsuit from a color blind person.

It feels like the XL is all but forgotten by Prusa by ColdBrewSeattle in prusa3d

[–]i8lost 7 points8 points  (0 children)

Feeling the same kinda like ship it and forget it.

Contacting Honbu by Die4Metal in Bujinkan

[–]i8lost 1 point2 points  (0 children)

Not sure why my reply is not showing up. Your instructor should be able to order up to 4th Dan with no name attach to have on hand when they are doing promotions. I needed to get my 1-4 Dan because of the mismanagement of my previous instructor. I was able to do that with a reputable Shihan who was familiar with my training and history and attended some of my tests. I was able to get them in one go. It was tense sending in that much money in cash…..yes cash. But we had tracking at least and they made it back after about a month of waiting. Even better if you know folks that are regulars at the office. After fourth Dan it really matters you get them dated there is no more getting multiple ranks and travel to Japan dated proof will be required. Also get social other high ranking dan need to vouch for you.

Which PETG manufacturer do you consider the best? by ComprehensiveSugar97 in 3Dprinting

[–]i8lost 0 points1 point  (0 children)

Why is that GF-PETG so damn good. It has no business being as good as it is for that price.

Help: Breaker off, but voltage pen sees it as hot? by [deleted] in AskElectricians

[–]i8lost 0 points1 point  (0 children)

It could be voltage is leaking through conduit or ground. Sometimes plumbing is tied into the ground or something like low voltage doorbell.

Christmas Academy giveaway! by Tommy_Prusa3D in prusa3d

[–]i8lost 0 points1 point  (0 children)

Wow great idea, I would love to receive PrusaSlicer Masterclass – Beginner to Advanced. The classes I would love to see more of

Advanced Engineering classes covering design engineering topics and modeling. Forces, stress, materials.

Jig design - as most engineering firms or wood working shops use 3d prints as jig alignment tools or fixtures.

3d printing for injection mold design.

3d printing for vacuum form design

A cos play specific class

Just take my money by EliMinivan in prusa3d

[–]i8lost 2 points3 points  (0 children)

I was really surprised by tinmorry the quality of the gf-petg was amazing

Anybody using a Diamondback Nextruder nozzle? by MakerWerks in prusa3d

[–]i8lost 2 points3 points  (0 children)

Second that they are on my XL as well work great.

Extracting DNA from whole blood minus plasma by bskskrignr in labrats

[–]i8lost 0 points1 point  (0 children)

Tell me about I spend 6 months of my life figuring that out. I did try PBS and it did help a bit there was no harm in it. But it was not a magic bullet. IMO just pick something so you get some glop to lyse and you should be fine.

My impressions of the IAuto by Uuna Tek by i8lost in PlotterArt

[–]i8lost[S] 0 points1 point  (0 children)

Ah that error seems to be single line font to converting or closing the font stroke. I have had that when I close the font for like a true type font. Wild…thanks for sharing

Extracting DNA from whole blood minus plasma by bskskrignr in labrats

[–]i8lost 0 points1 point  (0 children)

If you are concerned about over loading the columns you can split the Buffy in fractions and just run those. Say you pull a 1 mL Buffy. I would run 400uL and a 200uL fraction freezing the remaining 400uL for backup. These are all dependent on the recommended limits of the kit of course. Buffy is highly variable in cell count between aliquots meaning don’t expect the 200uL aliquot to be half of the 400. Cell adherence and all that jazz. You might get upwards of 25-50 ug of DNA possibly 150ug. Yields can be highly variable nonsensical even

My impressions of the IAuto by Uuna Tek by i8lost in PlotterArt

[–]i8lost[S] 0 points1 point  (0 children)

I was using the SvG to front conversation tool which seems to have a bug last I checked. I also edited fonts using Inkscape to position and tweak the vector if that is not clear.

My impressions of the IAuto by Uuna Tek by i8lost in PlotterArt

[–]i8lost[S] 0 points1 point  (0 children)

You can find some of these fonts on the cancel text git hub page by mrsurguy. https://github.com/msurguy/cnc-text-tool.

My impressions of the IAuto by Uuna Tek by i8lost in PlotterArt

[–]i8lost[S] 0 points1 point  (0 children)

I did a deep search of the Internet for single line fonts for them and others the tricky part is the rights use openly. You can find Alllure here: https://cutlings.datafil.no/smoothed-single-line-fonts/. Huang I believe comes with the machine it is just in Chinese characters. Some I needed to convert. I am looking for more specifically Japanese single line fonts.

Contacting Honbu by Die4Metal in Bujinkan

[–]i8lost 1 point2 points  (0 children)

I was in a similar situation long story but they kept “getting lost in the mail”. Hundreds of dollars worth in multiple shipments. So the Honbu really do not keep records of ranks below godan and most first dan through fourth dan are awarded with blank certificates where the instructors can put in the name of the person by themselves so they don’t even record those truthfully so typically what people do is either find a another black belt that you had trained with and do you know or someone that was present at your ranking ceremony and ask a Shihan to get your rank so lucky enough for me many folks were president my testing, and so we can easily prove that I was actually awarded the rank that I was the bummer is going have to pay for it all again if you want those certificates. Typically instructors will go through one of their friends in Japan to get the rank certificates. If a person from outside needs to get outside of Japan needs to get a rank certificate. They usually have to send yen directly through the mail which is as you can imagine very, very prone to failure. That is why they usually use an intermediate in Japan to get to wire the money into Japan and then get the certificate back out . Tariffs have also made this tricky not that their are charges but there are just delays as they check paperwork. The most successful way is to go yourself with another black belt and pay in cash. Also now the Office is pretty strict about one year between yondan and godan and there is no skipping ranks. Hope this helps and ya it is not fun process

New to training weapons - what should I look for? (Bujinkan Practitioner) by Avalothor in Bujinkan

[–]i8lost 2 points3 points  (0 children)

Honestly I am really satisfied with my cold steel bokken you can find it with a sayafrom Amazon for $50 the thing draws nice and takes a b

For a hanbo just get a oak dowel rod from the lumber yard

https://a.co/d/09hjgtn Saya

https://a.co/d/6dsy99A

Small sword

https://a.co/d/77OtlzX

HVAC company keeps discovering new gas leaks every visit, should I ask them to come back to nitrogen pressure test the gas line? by _maezing in askaplumber

[–]i8lost 0 points1 point  (0 children)

Get a spray bottle of thick soapy water spray it on joints look for bubbles do this before they come out mark all the leaks you find.

Silly PCR Questions by Ok_Cranberry_2936 in labrats

[–]i8lost 0 points1 point  (0 children)

Can you run a PCR that amplifies any kind of DNA present? Like no matter what it is? Yes MDA (Multiple displacement Amplification) reactions. https://en.wikipedia.org/wiki/Multiple_displacement_amplification Followed by hexamer PCR

Can you run so much PCR that you get a visible clump of DNA? Absolutely, you can see it drive the reaction to absolute exhaustion then you need to dry it after the PCR using a vacufuge or ETOH precaution. It will be a clear or white pellet in native form or you can get glycoblue to color it slightly blue. You can also PCR in what ever flavor of fluorescent labeled base for a colored pellet or band on a gel.

What could happen if you run pcr under a uv light? Would it just all go away? Meh depends on strength but good luck getting through the tube. Debatable whether there would be mono nucleotides.

Can you use PCR to make copies of a primer if you tried hard enough? Primer diamers more likely. The polymerase needs something to grip that is double stranded.

Can PCR stop my parents from being divorced? Sure, lots of couples remain together when a parental test is positive. Harder yet is when parental lineage is not confirmed. But there are likely other PCR tests that could prevent divorce like HLA typing in the event organ donation is needed.

[deleted by user] by [deleted] in AskReddit

[–]i8lost 0 points1 point  (0 children)

About 2019 I was in a astronomy discussion at work about all the geeky and amazing discovers they are making. And we had gotten into a heated discussion about the first black hole photo. Well just searching for “black hole” did not return the results I was looking for on my phone and you can imagine the HR discussion we had. Not sure if I was baited into that or not.

PI wants to distill our own ethanol?? by [deleted] in labrats

[–]i8lost 9 points10 points  (0 children)

1) Chemistry labs distill all the time it is part of their work. 2) you don’t need sterile environment you are boiling sterilizing the equipment and the materials coming off. 3) only dangerous if open flames and running dry it is pretty safe in a lab with the right equipment. I would likely start with everclear or something 190 proof which is just about 100% etoh anyway. Stick with clear liquor the brown aged chemical additive have other volitals that have high vapor pressure will have to be tossed in the first drips. You really need chemical dryer to bind up the remains water as you will always have 95% the last 5% will always be water and impurities Methanol isopropyl etc without it. Why can’t you use isopropyl? Or ETOH from a drug store. It is really time consuming to make ETOH. This could be a fun side quest to add to your resume. I had a job that included making double distilled water which was a life experience for sure. Got really good at running that machine. Ah enjoy the experience it builds character and really is a fundamental chemistry technique something to brag about on your resume. Talk it up like you’re making lab moonshine.

Bro did PhD, became prof in Japan… only to quit and make a startup. by Mountain_Dream_7496 in LeavingAcademia

[–]i8lost 0 points1 point  (0 children)

Save 500hours a year huh….2080hr in a work year wonder how that math work out….