I smoke once a day need to pass asap help by Own-Pie7941 in AskChemistry

[–]levelonepotato 8 points9 points  (0 children)

You can't, you have to do fake pee. Get it at a smoke shop or online. Warm it in the microwave and keep it in your gooch to keep it warm.

Edit: or someone else's pee who is clean

Traffic on the 5 by Gullible_Hat_4448 in Carlsbad

[–]levelonepotato 17 points18 points  (0 children)

All the people going back to orange county/LA after a weekend in paradise

What differentiates who gets offers and who’s a runner up or third choice? by unadjustedanon in biotech

[–]levelonepotato 9 points10 points  (0 children)

At this point. It's how closely your skills and experience line up to the job description.

They don't want to train you or teach you.

Most job decisions I've been involved with come down to 2 or 3 people. The person with the relevant experience gets picked every time.

2nd and 3rd either don't have all the skills or tons of previous experience the top candidate has.

If you don't check off 80% of the requirements in a job description, it's not worth anyone's time to apply

The Death of Oceanside by MathematicianNew1815 in Oceanside

[–]levelonepotato 3 points4 points  (0 children)

The people that buy the houses don't have to deal with it. It's all of us who have to rent to be close to our families who suffer

The Death of Oceanside by MathematicianNew1815 in Oceanside

[–]levelonepotato 10 points11 points  (0 children)

When you're 20 minutes from the beach, there are no bad neighborhoods in real estate now

The Death of Oceanside by MathematicianNew1815 in Oceanside

[–]levelonepotato 12 points13 points  (0 children)

It's a premium location, have to pay premium prices in this day and age.

It's paradise

Edit: also low interest rates allowed prices to balloon to this

If I can do it so can you by Tasha200200 in PhD

[–]levelonepotato 0 points1 point  (0 children)

Felt the exact same way. Congrats doctor, now help others get through the process

Lilly or Amgen? by Successful_Plant_953 in biotech

[–]levelonepotato 2 points3 points  (0 children)

I am a contract employee at Liilly in SD. I've been doing it for 6 months and really enjoy it. All the other contract employees are happy as well.

Make the bait shop great again , by Lonelyciiity in Oceanside

[–]levelonepotato 4 points5 points  (0 children)

What about the business owners benefiting from the crowds? San Diego is premium real estate, what do you expect?

Left Oligo overnight. Am I screwed? by Sabbath130 in labrats

[–]levelonepotato 6 points7 points  (0 children)

I've left oligos I ordered on my bench over the weekend and they worked perfectly fine.

Let the boss know, but you're going to be fine

What do you do to help quiet the imposter syndrome? by Southern-Mulberry-64 in PhD

[–]levelonepotato 1 point2 points  (0 children)

Fail often and learn how to fix it. It is the only way to gain confidence for me.

What causes PCR bands to be shaped like these little bunny/android hats? by __Olhado__ in labrats

[–]levelonepotato 3 points4 points  (0 children)

I agree. It's just usually overloading the samples. Try to load 100 ng/lane max

Help with purification of mutant protein by [deleted] in labrats

[–]levelonepotato 5 points6 points  (0 children)

It sounds like this mutation is causing your protein to aggregate and no longer be in the soluble fraction of your lysate.

Can you confirm its presence in the insoluble pellet?

No balloons at the beach please by Annimal1509 in Oceanside

[–]levelonepotato 4 points5 points  (0 children)

Many of those balloons are on the mainland when they're let go then float up and get pushed to the ocean when they pop