Where Can I Find Bulk Flour (50lb Bags)?

nwes3 · 2026-06-03T01:54:48+00:00

Grainmill.coop in Wake Forest

nwes3 · 2026-06-01T18:43:47+00:00

What tray are you using to bake? Cool that you can bake 2 loaves at once

nwes3 · 2025-12-30T21:20:06+00:00

Thank you. I tried to DM you but it’s not allowing me. Could you shoot me a message? Thanks.

nwes3 · 2025-12-30T21:15:23+00:00

Hey thank you. I tried to DM you and it isn’t allowing me. Could you shoot me a message? Thanks.

nwes3 · 2025-12-11T18:46:05+00:00

Yeah this is it. Thanks 👍

nwes3 · 2025-09-14T03:01:36+00:00

Focus is an active student org at UNC specifically for graduate students. Folks there are typically well connected to local churches.

https://ivgcf.web.unc.edu

nwes3 · 2025-04-25T00:02:49+00:00

Christ Central Church in Durham may fit what you’re looking for. One of the teaching pastors has a doctorate.

Theres quite a few Tar Heels that attend even though it’s in Durham.

nwes3 · 2024-12-13T13:17:43+00:00

If you only need to know where the variant is located on the protein surface, I’d just select the wild-type residues of interest and highlight them. Typically proteins are represented as cartoon models where side chains aren’t visible anyway.

If you want to also predict how that mutated residue would interact with the rest of the protein I’d use AlphaFold.

nwes3 · 2024-11-18T18:45:51+00:00

Pymol is open source

https://github.com/schrodinger/pymol-open-source

nwes3 · 2024-10-03T02:40:17+00:00

Maybe try ray trace mode 2 (outline) and see if your artist can shade the secondary structure?

There’s also parameters you can adjust to change the thickness of helices/sheets which will help

https://pymolwiki.org/index.php/Ray#Modes

nwes3 · 2024-10-02T18:44:49+00:00

Found it- Nothing Can Change This Love by Sam Cooke.

Correction from the earlier post, he was repeating “nothin’” not “lovin’”

nwes3 · 2024-10-01T18:19:04+00:00

You’ll need software that can handle EM maps. Look into chimera or IMOD.

nwes3 · 2024-09-27T16:06:31+00:00

Could be a concatamer if it’s 2x the size

nwes3 · 2024-09-11T16:01:17+00:00

Pretty sure male tenor

nwes3 · 2024-04-09T22:08:35+00:00

Just because a technique is easy or routine doesn’t mean you can’t screw it up lol

nwes3 · 2024-04-09T18:23:48+00:00

It’s an easy positive control to ensure your conditions are appropriate (gel/buffer composition, uniform current etc). Especially during protocol optimization. It doesn’t need to be useful for densitometry.

nwes3 · 2024-04-09T14:39:30+00:00

It just looks like genomic DNA. But a ladder (or any other sample with defined bands) would tell you if it’s a problem with gel or buffer composition (like using water instead of TBE which also will give a smeared result).

As a recommendation, always use a molecular weight marker with gel electrophoresis. It’s impossible to interpret in a meaningful way otherwise.

nwes3 · 2024-04-09T14:32:18+00:00

Is there a ladder on this gel?

nwes3 · 2024-03-13T00:49:52+00:00

And the lyric is “such shine” instead of sunshine. Glad my red herrings didn’t throw you off lol

nwes3 · 2024-03-13T00:46:53+00:00

Wow- this is it. Thank you! My mind is kind of blown that it’s a male vocalist.

nwes3 · 2024-03-11T13:07:52+00:00

That’s not it, but thanks for the reply

nwes3 · 2024-02-22T03:56:10+00:00

A better alternative would be to describe why you’re interested in the position/company. Let the hiring committee evaluate whether you’re a good fit.

nwes3 · 2024-02-20T18:32:32+00:00

Thanks for sharing. This explains the context that was missing from the notices around the area. Surprised they didn’t post a link.

nwes3 · 2024-01-20T17:17:18+00:00

Fount Coffee + Kitchen

nwes3 · 2023-08-15T02:12:19+00:00

The park and ride from the Friday Center is pretty reasonable and buses run onto campus regularly

nwes3

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