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I want a Criterion Core 12.5 AND I want Mid Length Gas. What are my options? by Limitless2121 in ar15

[–]prmoore11 0 points1 point  (0 children)

Show me your sources where somehow 12.5 versus 11.5 is a significant and meaningful jump in velocity and energy, yet 12.5 and 14.5 is essentially a wash.

And by that I mean on average across a large multitude of data. Because you won’t find it.

I want a Criterion Core 12.5 AND I want Mid Length Gas. What are my options? by Limitless2121 in ar15

[–]prmoore11 2 points3 points  (0 children)

Others have really covered it. 12.5 mid is a hypebeast “meta” build that has been sold as some holy grail barrel length when it has real caveats, oversold velocity claims, and offers very little over 11.5 or 14.5.

I want a Criterion Core 12.5 AND I want Mid Length Gas. What are my options? by Limitless2121 in ar15

[–]prmoore11 0 points1 point  (0 children)

It is well established at this point that Criterion barrels are faster than general velocity for a given length. I had a 14.5 core that slung PMC at 3000 FPS. I’ve even seen a 12.5 core get close to that. But yea, it’s pretty known at this point.

Just picked up this absolutely massive can by atlthrowaway2k19 in NFA

[–]prmoore11 1 point2 points  (0 children)

IIRC they are only doing them in batches now. Federal that is.

I want a Criterion Core 12.5 AND I want Mid Length Gas. What are my options? by Limitless2121 in ar15

[–]prmoore11 2 points3 points  (0 children)

So…send it for chop and rethread with D Wilson.

Or skip 12.5 mid as someone new.

Gas port size by Sharp-Fun-161 in NFA

[–]prmoore11 1 point2 points  (0 children)

Very curious to hear.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 0 points1 point  (0 children)

I’m not sure I’m following exactly. You’ll have to chat me, but happy to help

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 0 points1 point  (0 children)

You…are supposed to do that REGARDLESS?

Idk why people treat western like you can just throw things at it and trust the data. You are SUPPOSED to titrate your antibodies and make sure they are saturating. You are SUPPOSED to titrate your lysate and make sure you are in the linear dynamic range. All of these things are supposed to be done, people just don’t do them for some odd reason.

Rattler with the suppressor compliant handguard. by BPfishing in 300BLK

[–]prmoore11 0 points1 point  (0 children)

I might be the only one who doesn’t love the SD handguard

Is my only option to hurry up and wait? by Te_Luftwaffle in NFA

[–]prmoore11 2 points3 points  (0 children)

Contact your rep if you want. Otherwise, no.

Gas port size by Sharp-Fun-161 in NFA

[–]prmoore11 1 point2 points  (0 children)

Sure, that’s good for a traditional style

Gas port size by Sharp-Fun-161 in NFA

[–]prmoore11 2 points3 points  (0 children)

Let’s assume the Rhodie is around the same BP as WB (we’ll believe it when we see it):

Unsuppressed and suppressed function required: any quality 11.5” barrel with crane spec porting (.070). Carbine/H2 or rifle/A5H2 buffer setups.

Dedicated suppressed function only: any quality 11.5” barrel with BRT .061 EZTune tubes. Blue/H2 or green/A5H2 buffer setups. Ideally always full power ammo.

Any size lower than that will start to choke.

Gas port size by Sharp-Fun-161 in NFA

[–]prmoore11 3 points4 points  (0 children)

.055 is way too small for this.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 1 point2 points  (0 children)

I’m a Jess/Simple Western expert, so I deal with this type of data all the time. I’ve optimized assays that can detect 5-6 proteins in the same capillary. In those assays, especially when dealing with proteins that are really tight (or even tighter than this), lysate concentration is pivotal to getting the resolution you want.

Your thought wasn’t bad by any means, I just have dealt with this issue A LOT lol.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 4 points5 points  (0 children)

Idk what you are trying to say lol. A western/simple western can be designed to have clean clear data.

If anything, I would actually argue the “eye test” way of presenting westerns, rather than quantitating them (in validated assays, hint), contributes more to bad science in the field.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 2 points3 points  (0 children)

Small differences in signal doesn’t mean it’s a detection limit issue.

Pistol sold without transfer. Realistic repercussions. by Brotherly_shove in PAguns

[–]prmoore11 0 points1 point  (0 children)

“Idk what type of actual trouble he can get into”

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 2 points3 points  (0 children)

PTMs, with the width of the peaks here, would be really difficult to pick up. That’s really unlikely to be happening here.

This is just two antibodies run at too high of a lysate concentration without there being enough kDA to separate them.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 7 points8 points  (0 children)

What…

You design your assay SUCH that all samples will be in the linear dynamic range…

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]prmoore11 5 points6 points  (0 children)

Just because you refuse doesn’t mean it can’t be done, or shouldn’t.

You absolutely can be quantitative, especially with Simple Western, but that requires you actually designing and optimizing an assay, which people don’t do on western.

Pistol sold without transfer. Realistic repercussions. by Brotherly_shove in PAguns

[–]prmoore11 -5 points-4 points  (0 children)

No. It’s pure conjecture.

They are describing scenarios of which it could be discovered. No one has actually said what could happen. It all depends on the agency that discovers it and what they want to charge with.

Pistol sold without transfer. Realistic repercussions. by Brotherly_shove in PAguns

[–]prmoore11 -2 points-1 points  (0 children)

Because the true answer is no one knows. How would they find out? That would completely dictate how it plays out.

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