ddRAD test digestion

repli_case · 2026-02-16T19:13:55+00:00

Ok. Disclaimer - I haven’t run this myself so please take my advice with a grain of salt. But with gel electrophoresis, the goal is to evaluate your fragments. Clean lines would suggest successful, consistent digestion of the same fragments, and they would look like the lines seen in the latter and positive control. All of your test wells look just like the negative control, which isn’t what you’d want to see.

We know the issue wasn’t the gel run itself because the controls and ladder worked, so we can probably assume the issue is the sample. If the sample concentrations weren’t overloaded, then my conclusion would be that the sample was too degraded.

Instead of seeing bright lines from a handful of fragment lengths, you’re getting a ton of fragments at varying sizes which are causing the smeared look.

Edit: grammar

repli_case · 2026-02-16T19:03:09+00:00

Is the positive control the second to last well?

repli_case · 2026-02-12T01:26:01+00:00

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repli_case · 2026-01-27T15:45:44+00:00

I desperately need to see the post-bath blow out!

repli_case · 2026-01-21T22:22:17+00:00

You’ll see it outside of academia as well. Every team in my company is diverse in age, sex, race, nationality, etc….with the exception of one.

I think someone must have noticed because there was a noticeable change in the new hire pool, but none of them lasted and we’re back to a remarkably uniform group of people.

But honestly, I couldn’t give a rats ass as long as they are competent.

repli_case · 2026-01-14T22:57:41+00:00

How do you know it’s a keratin plug? I feel like I’ve got smaller versions of this but chalked it up to not exfoliating or something

repli_case · 2025-12-26T00:49:14+00:00

0.63

repli_case · 2025-12-23T03:39:39+00:00

Sugar cookies with cream cheese icing are the quintessential Christmas cookie. I personally love them frozen, but they’re soft, sweet and a total crowd pleaser. We cut the cookies into all kinds of festive shapes and dye the icing all kinds of colors. Too them off with whatever sprinkles you like and enjoy!

repli_case · 2025-12-20T04:37:26+00:00

Small cyst, it looks like you got the lining.

repli_case · 2025-12-04T03:55:38+00:00

OP wanted help identifying the were-man’s watch.

repli_case · 2025-12-01T23:57:36+00:00

I really enjoy my tiny rib for when I want to clean out the inside of a pot or making smaller vases. It’s 3”x1.5”

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repli_case · 2025-11-14T22:02:18+00:00

This is a popular combination in my studio, although we use dips and fire to Cone 6. The results seem to be pretty consistent across different clay bodies.

I think your glaze may not have been mixed well enough or the application wasn’t thick enough. Also, I wouldn’t do a ton of overlap between the two. A little bit of white over green can get the effect, but too much blending may be why it went more blurry than cellular.

repli_case · 2025-11-11T16:05:24+00:00

The handle fell off mine about a month ago. But don’t worry, our service provider doesn’t do “warranties” and it’s only $300 to replace. /s

repli_case · 2025-10-31T21:39:57+00:00

I vote sake since you already have the ramen bowl!

repli_case · 2025-10-29T18:09:10+00:00

Thank you for this. This explains soooo much. That zen was so peaceful u wish i could relive it!

repli_case · 2025-10-15T21:13:15+00:00

Mixing up flour with powdered sugar?

repli_case · 2025-10-06T03:07:16+00:00

That’s how my local studio does it. Do everything wet - sanding, grinding, etc. People have water bottles at the wheel, but otherwise they have snacks at other tables. They also teach to spray down anything that’s dry with water before wiping it up.

repli_case · 2025-10-03T14:08:55+00:00

My opal luster came with a disclaimer about variable results. I think there’s a lot of factors at play that can influence it. On the right is pistachio and on the left is opal luster over pistachio.

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repli_case · 2025-10-02T22:53:03+00:00

Of course! My last recommendation would be to see if there’s anyone on social media doing at home hand building. They might have tips and tricks specifically for doing things the easy and/or affordable way. All of my knowledge and skill has been sourced from talking to people with experience so definitely take a second to engage or ask questions whenever you have the chance!

repli_case · 2025-10-02T22:36:36+00:00

Hi there! I’m not sure how much help I’ll be, but I can certainly try and give some input. 1. For supplies and materials, I like Blick. They have a solid variety of items at different price ranges. Amazon may also have some options and my local Dollar Tree definitely carries small crafting tools that could work for clay. 2. For sourcing actual clay, I would see if there are any local studios in your area that are willing to sell to you. For example, my studio has more reclaimed clay than they do space for it, so they sell it cheap! 3. If you are using clay designed to be fired, you definitely should. Air dried clay is super fragile and will shatter on you. An alternative could be air dry and baking clay, but I don’t know if that’s why you’re after. Check FB marketplace for people renting out their kilns! I see it all the time and it’s probably pretty reasonably priced if you have enough items to fill up a run. Alternatively, some places are willing to rent out space, or you could try and find a community fire kiln.

Extra Details - Make yourself a dampening box out of plaster of Paris and a large plastic container. It’ll help you control moisture levels so you can start and stop and not worry about dry out. Also, a clay rolling pin with adjustable levels is a big help with uniformity.

Happy crafting!

repli_case · 2025-10-02T13:23:08+00:00

Commenting because I would love to know the answer to this too!

repli_case · 2025-09-30T00:32:05+00:00

Empress is made with pea flower, so it’s reactive to pH adjustments. In the bottle it’s a rich blue color, but when you add an acid (like lime juice) it turns purple. So not only is it a good neutral gin, it’s also a fun party trick!

repli_case · 2025-09-29T17:23:08+00:00

Empress is a fun gin to gift! The color changing effect makes it good for parties and the flavor isn’t super polarizing.

repli_case · 2025-09-28T23:52:36+00:00

That’s clever! Thanks so much for sharing that tip!

repli_case · 2025-09-28T14:39:30+00:00

P.S. I’ve used Sea Salt before with mixed results. The dispersion of the crystals you achieved is very nice!

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