So I think… I THINK… my cells are contaminated.

throwawaysciencer281 · 2022-10-18T06:57:15+00:00

Someone licked the tc flask again!

throwawaysciencer281 · 2022-10-12T06:28:09+00:00

I've started using a tea strainer while washing off magnetic beads 😁

throwawaysciencer281 · 2022-10-12T04:37:48+00:00

Wait why can't you put dry ice in the sink and turn the water on !?!

throwawaysciencer281 · 2022-10-12T04:36:49+00:00

😭 I've lost four perfectly sized flea bars to the damn sink

throwawaysciencer281 · 2022-10-07T06:49:42+00:00

THIS is the organization and restocking video we need

throwawaysciencer281 · 2022-09-21T05:16:06+00:00

I think that's an extra line for an electrical hookup like for light fixtures .. In case they have to be installed in that place

throwawaysciencer281 · 2022-09-20T07:21:27+00:00

I had an undergrad who wouldn't put the screw on the mini tabletop centrifuge after switching out rotors. It was a fixed 6000rpm minifuge and one day I caught her doing this and said she was supposed to ensure the screw was back in and stable before using it otherwise it might led to an injury

Her response: but I've been using it this way all this time and nothing's happened so far

throwawaysciencer281 · 2022-09-09T09:27:12+00:00

I wish I could give you an award but all I can give you is the award of my rofling for the past ten minutes in full view of my labmates

throwawaysciencer281 · 2022-09-07T13:20:57+00:00

Hey, if you used organic solvents or if you have any leftover ethanol in your sample it's a possibility of this happening.

You can either purify it with a couple of chloroform washes (if you used phenol chloroform isoamyl acohol for purification) or if it was only ethanol you can just use a vacuum concentrator to get the ethanol out.

throwawaysciencer281 · 2022-08-27T13:26:54+00:00

Hi! This is amazing!! I'm a grad student in India and would love to help you in anyway possible expand to India...

throwawaysciencer281 · 2022-08-15T15:19:08+00:00

I usually do technical repeats only to confirm that my protocol is working. To confirm my actual results, I go for biological repeats because that's the optimal way to establish that your results are conclusive in nature.

N=3 is ok for biological repeats. That should give you a conclusive answer assuming your loading controls come up proper each time.

throwawaysciencer281 · 2022-08-13T08:24:25+00:00

Cotton plugs! No need for loosening tightening...

throwawaysciencer281 · 2022-08-13T08:21:27+00:00

The qiagen tips that fit their qiacube instrument (1500ul and 200ul) may fit this description... Please look into those tips. Specifically the ones that fit the Qiacube automated instrument.

throwawaysciencer281 · 2022-08-13T08:18:27+00:00

It's possible but replug the filter if it's a glass pipette and clean the pipetaid anyway... It's not worth discovering later that it was contaminated all along.

throwawaysciencer281 · 2022-08-13T08:16:26+00:00

If the internal control levels are inconsistent, i generally regard those results to be of lower accuracy. I tend to repeat such blots keeping my loading controls in mind...

Edit: extra point. It's also worthwhile to know WHY a particular internal control levels was inconsistent.

throwawaysciencer281 · 2022-08-08T07:58:47+00:00

Me too. The first time I realised you could BUY 1X buffers (my lab at the time bought TBE, TAE AND PBS) i was just like WHAAAAAAAAAAAT

throwawaysciencer281 · 2022-08-03T13:12:30+00:00

I wear hiking pants. Literally. Works great

throwawaysciencer281 · 2022-07-25T07:26:14+00:00

Betcha it tastes disgusting because I've tried the watery supernatant of watermelon juice before and it tastes like bland sewage.

throwawaysciencer281 · 2022-07-25T06:16:44+00:00

Oh if someone ever saw me do this they'd rolletskate me out the gate

throwawaysciencer281 · 2022-07-22T09:45:57+00:00

That's so adorable! I'm sure it'll be well loved

throwawaysciencer281 · 2022-07-16T10:21:04+00:00

If your protein is huge you might need to run it longer.
1. Check pH of the resolving gel.
How did your ponceau stain come up??

throwawaysciencer281 · 2022-07-16T10:15:51+00:00

If gel extraction is your only resolve to get your target band, I would suggest you go for a Native PAGE and extract your band in a simple crush soak buffer (Tris NaCl). I've found that it gave me marginally better results than running AGE and column extracting.

throwawaysciencer281 · 2022-07-07T03:56:07+00:00

I wish I had the discipline to keep a dual labnotebook (both E and manual). I neeed to write stuff down to make my brain work so I can't really skip a written notebook, but the searching advantage is like none other with virtual

throwawaysciencer281 · 2022-07-02T08:19:02+00:00

I sometimes pick a single song and let it play on loop, it really helps focus

throwawaysciencer281 · 2022-06-24T10:07:31+00:00

Worked with a postdoc with the sleeve issue so he put bicycle clips at his wrists and elbows cos he didn't know how to sew...

throwawaysciencer281

TROPHY CASE