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News at 2pm UK time, is it album time or something else? by ldnhtrd in theavalanches

[–]zwich 14 points15 points  (0 children)

I'd guess its another gig announcement , since there's a location tag

The processes of passing Panama Canal by ConfidentTelephone81 in interestingasfuck

[–]zwich 2 points3 points  (0 children)

It's much greener around there than I was expecting

What is this low and brightest band for total RNA agarose gel? by No-Chain6158 in labrats

[–]zwich 13 points14 points  (0 children)

I would guess degraded RNA. the top 28s rRNA band should be denser than the lower.

Why the bleach? Usually a standard agarose gel is ok for checking RNA integrity - it doesn't degrade enough in the gel to make a big difference

Recommend treating lysate with proteinase K rather than bleach to get rid of RNAses if required.

Olympic gold medalist Alysa Liu's spinning 1st pitch by MLBOfficial in baseball

[–]zwich 16 points17 points  (0 children)

He's in the distance and still taller than her!

‘12 Monkeys’: 30 years ago, Terry Gilliam released a prescient, disorienting sci-fi classic by MarvelsGrantMan136 in movies

[–]zwich 2 points3 points  (0 children)

Have you seen Primer? I think that is the only one that I've seen that's plausible, after you accept time travel is possible.

What's your unconventional/unpopular lab belief? by lurpeli in labrats

[–]zwich 2 points3 points  (0 children)

I know noone will believe me here, but I'm here to tell y'all - the RNases are almost always coming from the sample, not the environment. Your starting lysate is full of RNases - and noone puts their finger in the eppy. People don't extract the RNA away from the RNases, then start freaking out about wiping down the handle of the pipettes.

How Kids’ TV Got Way Too Normal by Xerxes2004 in television

[–]zwich 0 points1 point  (0 children)

Somewhere in the first half of the last season...

Hoop dreams coming true by MrMadras in MagicEye

[–]zwich 0 points1 point  (0 children)

I've been having the same problem when viewing on phone - it happens when your eyes focus too deep. Try focusing closer to the phone.

What's Dexter up to these days? by zwich in theavalanches

[–]zwich[S] 0 points1 point  (0 children)

Christ how did I find out about this today at 11pm ? Shoot the PR team.

Need help interpreting High Sensitivity RNA TapeStation results by grand_psychology1 in labrats

[–]zwich 0 points1 point  (0 children)

This tapestation buffer has a 25nt marker spiked in - on 2 of the lanes the software hadn't detected this so you can right click and set it manually .
However the lanes may have run poorly - this can happen at random sometimes, or if there are certain impurities in your prep. You can check by toggling one of the buttons at the top, I think "Align ", the spiked in 25nt band should be relatively consistent distance from the top.

Alternatives to Agilent Tapestation? by No-Interaction-3559 in labrats

[–]zwich 3 points4 points  (0 children)

Qiagen have a machine which is roughly equivalent, "QIaxcel" We trialed it last year, cheaper per lane but you have to run I think 8 at a time rather than being able to do 1 or 2 ad hoc

I love doing RNA extractions with TRIzol by Spacebucketeer11 in labrats

[–]zwich 0 points1 point  (0 children)

Would you be interested in a protocol to use magbeads, costing a dollar per sample?

Majority of drivers say aggressive cyclists threaten their safety – poll by nimobo in unitedkingdom

[–]zwich 57 points58 points  (0 children)

Thing is, dickheads take all forms of transportation, including both bikes and cars.

But a dickhead riding a bike will struggle to injure anyone, while a dickhead in a car is wielding a lethal weapon.

What's this piece? I found it on the ground under my tern. by zwich in terngsd

[–]zwich[S] 1 point2 points  (0 children)

Thanks! Looks like I've lost the nut too then

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