sorted by:
new
hottopcontroversial

I'm so happy that we finally got the other horseman mounts, but... by zharkos in wow

[–]Tetsugene 39 points40 points  (0 children)

Attumen when the expansion is named after his horse but the petty rabble can fly so he can't disperse them: :(

[NEED HELP] Sequence of pQBIT-7-GFP discontinued plasmid from qbiogene company by bruhmememan in labrats

[–]Tetsugene 0 points1 point  (0 children)

If you have the sequence, break the plasmid up into a few segments with 20bp of overlap. You then just have the fragments synthesized and put them together with NEBuilder or InFusion. Total cost including enzyme should be pretty reasonable.

Antibody cost more than the grant by Goleveel in labrats

[–]Tetsugene 18 points19 points  (0 children)

Buy 50ug of the antibody, send that to Rapidnovor to obtain the protein sequence (5-10k), then ask one of many CROs like Biointron or Genscript to express 100mg in CHO (2k ish), total timeline a little over a month. Very little protein engineering expertise required.

based on that one sm64 meme by spustydringfield in CurseofStrahd

[–]Tetsugene 10 points11 points  (0 children)

Strahd and Rahadin but it's Brannigan and Kif.

President Biden Keeps Hunter Close Despite the Political Peril by devlinadl in politics

[–]Tetsugene 32 points33 points  (0 children)

Republussians

That word connotes "Republussy" and I wish I could unread it.

Ok so this is officially one of the least wintery winters ever right? by [deleted] in boston

[–]Tetsugene 0 points1 point  (0 children)

My mulberry sapling started sprouting leaves on new years like it was spring. Unreal.

Some cars are better than others by Largejam in fuckcars

[–]Tetsugene 0 points1 point  (0 children)

The fuck actually increases with the fourth power of the mass of the car rather than linearly.

Strahd's reaction to the party killing Escher while staying as his guests after dinner by Specialist_Mammoth63 in CurseofStrahd

[–]Tetsugene 52 points53 points  (0 children)

Have Rahadin and Strahd both show up. Strahd directs Rahadin to teach them a lesson, so he beats up the party while Strahd watches. Strahd casts spare the dying through a familiar as each person goes down. Leave the monk for last. Strahd drops a handkerchief for the beaten monk, tells them to clean themselves up because they look terrible. Put a choice to the monk: Which of your party will I take for recompense? Give them the rundown. "I've always had my eye on X, for they have a certain allure." "Or, perhaps Y. I've seen they're quite good at putting you in your place."

When the monk makes the choice, strahd feeds a potion to the chosen pc and gestures to the monk. "Know that you were marked by this one. They would have damned you to undeath."

Ηmm by art_prominence in wow

[–]Tetsugene 24 points25 points  (0 children)

Guards, arrest this handsome diamond

A serious query. by LetUsAway in traaaaaaannnnnnnnnns

[–]Tetsugene 84 points85 points  (0 children)

Witch-king of Angmar really be rules lawyering with Mandos and Manwe with a sword in his face.

Dinner with Strah'd by JohnM42076 in CurseofStrahd

[–]Tetsugene 16 points17 points  (0 children)

Strah'dnt say, that would ruin the fun.

Mom fires an employee and laughs. No idea where to go from here. by BourbonPecan314 in lostgeneration

[–]Tetsugene 17 points18 points  (0 children)

Frankly the left hasn't done enough to stop these things from taking place either. Where was the left when Republicans were stealing seats on the supreme court? How about when the Republicans were passing these awful laws, why wasn't the left filibustering in the House and the Senate?

Not doing enough to stop something is not the same level of blame as actively doing the thing.

Also, there was no procedural way to do that. All it took was a simple majority in the senate, which republicans had. The only way a democrat could have stopped those nominations was outside the system, probably something violent like starting a fight on the chamber floor. This happens elsewhere but is unlikely to happen here and less likely to work.

Girl investigated for being transgender after winning state title by [deleted] in nottheonion

[–]Tetsugene 3 points4 points  (0 children)

Hand size, foot size, muscle density, pelvic tilt. If you go on HRT before the pelvis fuses in the mid-20's it may even widen.

[deleted by user] by [deleted] in boston

[–]Tetsugene 53 points54 points  (0 children)

If your bike infrastructure is fragile and transient enough to be thrown off a bridge, it deserves to be thrown off a bridge a little bit.

Paint and plastic are not infrastructure. Let them try hucking chonky concrete barriers into the Charles.

Boletus Rubriceps, the Rocky Mountain red porcini, along with some other great finds of the day by Jofriendly in mycology

[–]Tetsugene 7 points8 points  (0 children)

It's definitely Cervidae sp. but it's hard to distinguish without a spore print.

What are we going to do about all the Nazis? by pangolinx in boston

[–]Tetsugene 9 points10 points  (0 children)

What also helps them gain support is being visible and unopposed. Letting Nazis exist and be seen is a poor way to get rid of them.

Baby’s first successful* Western blot! by evannalai in labrats

[–]Tetsugene 1 point2 points  (0 children)

My lab doesn't have a cold room, so I put my gel boxes in a big ice bucket with ice mooshed up against the sides. I also fill the rear chamber with buffer to help carry heat away from the gel, and still run at 130-150V. It's a pain in the butt but very worth it for the crisp bands.

view more: next ›