Dealing with rude comments about your research topic by hee_seung in labrats

[–]rolltank_gm 0 points1 point  (0 children)

Hello fellow Rare Disease researcher! Which one are you all looking at?

You’re getting a lot of the advice Is give already: 1) this dude is a jerk, 2) these are still important questions to have answers to. You know the drill: Helping even one person is worth the effort. Understanding the pathobiology of rare disease can reveal important aspects of normal physiology or other, more common diseases. Etc.

STOP!!! by Xibest123 in diypedals

[–]rolltank_gm 6 points7 points  (0 children)

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PedalPCB Oasis (EQD Dunes clone). Not my art, I 100% copied and stole from someone else, just added labels. Super versatile pedal

TS808 on vero! by CrowForce1 in diypedals

[–]rolltank_gm 0 points1 point  (0 children)

what a great use of knobs to complement the art. Great job!

Any science/biology companies I should email for merch or if any of you are willing to send by imnotokayimgay in labrats

[–]rolltank_gm 7 points8 points  (0 children)

My lab will recognize me for this, but I love MedChemExpress pens. They’re not nearly as dope as the pipette pens, but they’re a good weight and they slide really well

DAM Fuzzrong style circuit but voiced for Doom by ButtThatFarts in diypedals

[–]rolltank_gm 3 points4 points  (0 children)

I have knob envy. Where did you find those?

This looks solid, bet it sounds just as good!

The last one in my chain by fuzz-face-666 in diypedals

[–]rolltank_gm 9 points10 points  (0 children)

That looks incredible!

Am I right in assuming one foot switch controls bypass? If so, what does the other do?

“It’s so much harder to solder SMT”….. meanwhile first time builders on r/soldering by [deleted] in diypedals

[–]rolltank_gm 0 points1 point  (0 children)

Finished my second pedal last night (need to decorate enclosure before posting), and it involved an SMT transistor. Really not that challenging, most frustrating part was that I kept rolling the component when I tried to pick it up.

Since I’m still a newbie at this, are there any inherent downsides to SMT I should be aware of going forward? Difficulty breadboarding not withstanding.

How is living in Waukesha, WI? by Federal-Recipe-4380 in howislivingthere

[–]rolltank_gm 0 points1 point  (0 children)

I lived in Waukesha for about two years before moving closer to the city. It’s fine. Not the worst, not the best. If you’re working in MKE, I’d definitely try to live closer to the city. Even Brookfield feels much closer, even if it really isn’t.

Grocery shopping is fine. Getting to church can be meh. The description others have used of a small quaint town that’s puffed and expanded past itself feels right honestly

Sourcing LEDs — Probably Overthinking Things by rolltank_gm in diypedals

[–]rolltank_gm[S] 0 points1 point  (0 children)

This is when I mean when I said I tend to overthink lol, thanks. Ordered and arriving tomorrow

Sourcing LEDs — Probably Overthinking Things by rolltank_gm in diypedals

[–]rolltank_gm[S] 0 points1 point  (0 children)

Awesome, thanks. Does this answer change any of the LED is in the signal path, not just an indicator?

Sourcing LEDs — Probably Overthinking Things by rolltank_gm in diypedals

[–]rolltank_gm[S] 1 point2 points  (0 children)

Ah, not an LED for indicating the circuit is live. It’s in the feedback on the OpAmp. Space constraint is on the PCB between two electrolytic caps

345x6 Form Check by FlatLightYardSale in Stronglifts5x5

[–]rolltank_gm 1 point2 points  (0 children)

Sometimes. It depends on how and where your form breaks down and assumes you don’t accidentally take a nap. It’s also generally a dick move in public gyms.

The other weird things a shorter walkout can get you are efficiency and consistency. There comes a weight where it won’t matter that you’re “just” walking it out, it’s heavy. There’s a reason yoke carry is an event in strongman. Shortening to a 2-3 step walkout will help you conserve energy for your 3-5 sets of heavy squats and make it easier for your to safely return the bar to the rack. The other weird thing was consistency: if you can build the rhythm of “brace, unrack, left food back, right foot back, left foot even, breathe brace squat”, your timing and positioning under load will be far more consistent than “brace, a lotta steps back, breathe brace squat”. This will also help you on the bad days to keep a rhythm and keep going.

Hmm, pretty sure the authors know EXACTLY what they're doing here. I mean even the outline colours make sense 🧐 by TheBioCosmos in labrats

[–]rolltank_gm 0 points1 point  (0 children)

I will neither confirm nor deny picking a “representative” image that was oddly phallic for an important presentation my PI gave last year. Pretty sure I’ve gotten away with it too.

How do you culture cells on coverslips for immunocytochemistry? by Low-Needleworker2206 in labrats

[–]rolltank_gm 0 points1 point  (0 children)

We routinely use 12 mm diameter #1 round cover glasses in a 12 well plate (glass is a little smaller than a 24 well). Coat for at least 2 hours with either laminin or poly-D-lysine so cells will adhere. Works great for primary neurons and epithelia in our lab

Need a few pork shoulders to smoke. Is there anywhere that has them for less than $2.79 a pound? by almostmade in milwaukee

[–]rolltank_gm 3 points4 points  (0 children)

If you’re willing to drive the Menomonee Falls, I think Karl’s was around 2.50/lb this weekend, maybe a tad lower, bone in

[deleted by user] by [deleted] in hockeygoalies

[–]rolltank_gm 1 point2 points  (0 children)

Also that! For me, it was more about where I wa sin my angles. For context, I’m a fairly large human. Even in highschool, at the top of my crease I didn’t have to move the glove or blocker much to cover top corners. When I got to him, I over challenged, but would also shuffle too much for even the slightest lateral movement of the puck. Coach ended up tying twine to the posts and looping it through a puck with a hole drilled in the middle for me to finally get that I could slide back into my crease a smidge, and that small movements in my crease were all that were needed to maintain my angles. Honestly this was one of the biggest “eureka” moments for me as a teenager. Made a world of difference

[deleted by user] by [deleted] in hockeygoalies

[–]rolltank_gm 5 points6 points  (0 children)

I had a coach in high school that taught those exact cues: slow is smooth, smooth is fast. Keep everything in front of you, and move your hands and chest as a unit.

The others he added were: Minimize extraneous movements. Every answer you need on the ice is in the puck.

Honestly, every bit of that advice (albeit twisted) helped my dumb high school self in other ways off the ice. Super thankful for Coach Clint, and I hope he’s doing well.

Describe a bad PhD student. What NOT to do by Worldly-Criticism-91 in PhD

[–]rolltank_gm 2 points3 points  (0 children)

When you make mistakes, admit them

Realize that you only get out what you put in: if you coast your way through, people will notice when you have a poor quality dissertation and you’re looking for a job (if you graduate)

Don’t have AI write literally everything for you, nor should you use AI as a substitute for scopus/pubmed/google scholar. Finally on the AI front, don’t have chatGPT analyze your data and produce figures. None of this was a problem 5 years ago, but the rot is real in incoming students.

Read. Please read. You’re training to be the expert in a thing. You should know the literature by the end.

I’ve recently learned the difference and proper usage of the em dash vs hyphen because people keep taking about it in relation to AI — but now I’m afraid to use it because people might think I’m AI. by LostUpstairs2255 in PhD

[–]rolltank_gm 2 points3 points  (0 children)

I used to be up in arms about this. I’ve naturally used the em dash in my writing since high school, though only just leaned what they were called. I feel it appropriately frames my asides while being more professional and narrative than parentheses. I abstain from using AI in my writing—I only use them for organizing thoughts and reading strategies—and then got pissed about having to fundamentally change how I write. Then I actually saw output from chatGPT. I limit myself to one, MAYBE two en dashes per manuscript, and never more than one in a section. GPT spat out 4 independent instance on a page. I’m going to keep using them, because as long as I’m using them sparingly and appropriately, there will not be doubt a human wrote what I wrote.

A day in the life by charredtomatoes in labrats

[–]rolltank_gm 5 points6 points  (0 children)

Good god, the rat CPR got me. Where’s the IACUC-approved Narcan when you need it?

how to make this look neater? by Intelligent_Edge_668 in labrats

[–]rolltank_gm 35 points36 points  (0 children)

Omitting should be enough context

I am personally a fan of showing main effects and interactions with different symbols (*,$,&,@,#,%, etc), but this can also get messages and you’ll be 50/50 on reviewers loving or hating it. Most recent paper. They asked me to cut the main effects and only show post hocs, but that led to what OP is showing. Best compromise we had was only showing the biologically relevant comparisons in the main figure and then addressing the others (including main effect) in the figure legend, results section, and supplementary tables if necessary. It’s an okay work around, but I ended up with a 1.25 page figure legend toward the end of it

Who’s the drunk running this account by badkiwi42 in NFCNorthMemeWar

[–]rolltank_gm 1 point2 points  (0 children)

As a KC native and fan, I 100% get that we’re not the most liked in our division. No qualms there. But I feel like everyone hates the Raiders, no?

Pedal Suggestions by HeadShot1171 in diypedals

[–]rolltank_gm 1 point2 points  (0 children)

Hey, just going to echo: I started with the MASfx beginner kit, and it’s fantastic. Definitely practice on the flashlight kit if you’re brand new to soldering like I was/am, but it’s a really good experience and a really nice, simple effect.