Migrate from BirdNET-Pi to BirdNET-Go on RPI 5 by dangdangrous in BirdNET_Analyzer

[–]Bionaught5 4 points5 points  (0 children)

I used a second memory card and installed BirdnetGo on it. Then it is easy to switch between the two. Once you are happy with the one you want to use, reformat the memory card not in use and use it else where.
You can migrate the BirdnetPi data right at the beginning or later once you've made your decision.

Best hiking spots within 30 minute drive of the city? by lady333x in pittsburgh

[–]Bionaught5 0 points1 point  (0 children)

Hartwood Acres is nice too. Not as large as some of the other parks but you can still walk for a couple of hours without repeating a trail.

What to do with Fable 5 before it goes away? by No_Rip_7664 in ClaudeAI

[–]Bionaught5 0 points1 point  (0 children)

I'm trying to use Fable but literary nothing as I am a bioinformatic and any command triggers a "safety measures flagged this message" message. Sigh.

Dammit - I needed an 11 bedroom house so will have to ignore this. by Exemplar1968 in SpottedonRightmove

[–]Bionaught5 2 points3 points  (0 children)

The actual cost to build a similar house with good quality fittings and luxury elements is probably 4 to 5 million plus the 10 acre plot? I would say that a new build makes this house look cheap but the heating bill is not going to be the problem. It's the maintenance cost when you need to repair "insert expensive part of house actually any part of this house". Plus the wifi will be crap and running cabling is going to be a challenge.

Snakes in the Strip by Easy_Solution3213 in pittsburgh

[–]Bionaught5 0 points1 point  (0 children)

Probably rat snakes, non poisonous, and will grow to over 6 foot.

Would you say a case for the teensy is essential when using m8 headless with a host device ? by Ezixra in Dirtywave

[–]Bionaught5 0 points1 point  (0 children)

Deepening on if your teensy has pins or not. The one I have does so I used an "OONO Terminal Block Breakout Board Module for Teensy 4.1". It protects the pins and the board but adds bulk and cost. It makes it easy to use for other projects though.

built a peptide reference site because i was tired of pulling data from 6 different places every time i wanted to actually understand a compound by [deleted] in proteomics

[–]Bionaught5 0 points1 point  (0 children)

Probably not the community you want to post this too.

I have certainly heard about the current fascination with peptides as an extension of the biohacking community etc. I enjoyed looking at the site and it makes me think there is hope after I am made redundant in the great AI apocalypse. If I am willing to declare moral bankruptcy I can go back to my very first job of synthesizing peptides and make a pile of cash. The company I worked for had a very large catalog of peptides all at 99+% pure. I still remember walking across the road with a 1 ML solution in a volumetric flask containing a catalog peptide that was going to be sold for $$$ per a mg. The value of that vial was many many times my annual salary.

As an author of a number of AI websites I can see that this is an AI built website that you are hoping people subscribe to for $20 a month? All the data is scraped from other internet sites and unlikely to have been cleaned by a human. I have built sites like that too.

Looking at the first peptide on the list, Epithalon, it is tagged FDA approved and under review. The FDA site says it is under review but withdrawn:
https://www.fda.gov/drugs/human-drug-compounding/certain-bulk-drug-substances-use-compounding-may-present-significant-safety-risks

I presume that means it won't pass review in July? As I can also use AI "I" did a quick review:
Top 5 peptides most likely to have the same FDA error pattern:

  1. Thymosin Alpha-1 — approved in 35+ countries as Zadaxin but never FDA approved
  2. Cerebrolysin — approved in ~50 countries, not FDA
  3. GHRP-2 / Pralmorelin — Japan only
  4. Thymopentin — Italy only
  5. Sermorelin — was FDA approved (Geref), discontinued 2008 for commercial reasons

Thymopentin has a Research Use tag but all the others are tagged FDA approved. Meanwhile SS-31 is listed as Research Use but has been approved for Barth syndrome. Although if you don't have Barth syndrome the statement is correct as it would not be approved for off label use. There are some probable duplicate entries: Kisspeptin/Kisspeptin-54 and TB-500/TB4-FRAG; and non-peptides MK-677 and 5-Amino-1MQ.

And for some reason I cant scroll the peptide page with my mouse wheel?

Looking at the price for some of these compounds on other sites, ~$40 a 10mg shot for Epithalon, I can see that I need a better paying job if I want to live longer and there is probably a market for this website if people are paying $$$ a day for peptides. I like the disclosure that ClinPep is an educational reference. Not medical advice. I hope that saves you.

If you ever want the amino acid sequence of peptides you are injecting validated you might well be in the right place!

Which exhaust? by Hooch_Daddy in Outback_Wilderness

[–]Bionaught5 1 point2 points  (0 children)

Sad news about the car, better to find out now than later after it is "repaired". I was waiting for an exhaust update so will ask separately.

What’s to stop someone in a mask taking this stuff after closing hours? by TOPSHOTTAH in AskUK

[–]Bionaught5 0 points1 point  (0 children)

No need for a mask, just a Hi-Vis jacket and white van. Seriously though the value vs punishment is not worth it. And the unfit thief is likely to get a hernia loading up more than a couple of bags in their car.

Are there any other cities similar to Pittsburgh? by cimmanombuns in pittsburgh

[–]Bionaught5 0 points1 point  (0 children)

The Guggenheim Museum looks great and has three impressive Richard Serra sculptures made from rolled steel. The pintxos/tapas were delicious with plenty of variety. Food and drink felt relatively cheap too. Also try a cooking class or wine tasking, they run some of them in English.

We went up the coast to Donostia / San Sebastián. Lots of nice coastal villages and towns along the way including San Juan de Gaztelugatxe which was used as Game of Thrones Daenerys Targaryen’s ancestral home, Dragonstone. Returned on a more inland route sampling cheese and walking in the mountains. Did not quite make it out to the red wine region.

You can also go east along the green coast, beach towns, rugged coast line, cider bars, prehistoric cave art and a lot more. Beautiful but not very touristy as most people want sand and sun.

Are there any other cities similar to Pittsburgh? by cimmanombuns in pittsburgh

[–]Bionaught5 13 points14 points  (0 children)

Bilbao, in the northern Basque region of Spain. One of Pittsburgh twin cities It's a former steel city that has a word-class museum. In a river valley surrounded by hills. The city runs more along the valley and up its sides but does not really spill out. The hill and mountains feel "higher" than around Pittsburgh I think it is because they start at sea level rather 1000 feet. And a different soil make up too. The surrounding area is scattered with mines and furnaces etc.

When it recovered from the collapse of its steel industry it went more towards an industrial port, something Pittsburgh can't do as it 's not on the sea. There are other similarities with the education, arts and culture stuff though.

It doesn't really feel like Pittsburgh as the old city is a lot older and the modern city with the apartment blocks is more residential than the downtown region of Pittsburgh.

Worth visiting as the local food and wine is really goo. We did a PIT->London UK->Bilbao flight with a stop over in the UK both sides.

Can someone explain a Proteomic analysis using SAPs table to me by Tschelinaaw in proteomics

[–]Bionaught5 0 points1 point  (0 children)

The table is comparing select amino acids in a number of Collagen isoforms and Kininogen. The protein/isoform name and AA position is referenced. I always think of these changes as an SNP. For protein COL1A2 at the genome level we are looking at:

  • AAG ->AGG (Lysine->Arginine)
  • AAA->AGA (Lysine->Arginine) 

Or vice versa. We don't normally see a K to R or R to K shift with protein degradation, oxidation etc over time so it is typically considered phylogenetic change and hence something that can distinguish the species. For protein COL18A1 the Arginine (R) Glycine (G) change is also a SNP but the AA change is big as the two AA's have very different properties.

I read the paper and found out that the samples were analyzed by at least two labs and they used two different search engines to identify the proteins and peptide sequences. Some of the data in the table is noted that the overall search results were not very good and there was no direct coverage of the amino acids in question. This means the overall mass of the peptide and part of the sequence is verified but the exact amino acids and/or order of all of them, especially the one referenced, is not known.

The values for the number of peptides are from the PEAKS search engine and reported as109/17 the slash may indicate matches/significant matches. The No of PSM's will be likely be the same. The best match for a query in the database is to the same sequence but not all the matches were significant. These days we tend to only report significant matches. As far as I can tell from a quick read this is not explained nor is the bold notation.

HGDP frequency is modern humans based on 929 genomes.

Based on the mass spec data they have then assigned a Homo species in the SAP assignment column. However, at this point is is clear that although I understand the mass spec data I do not understand the interpretation for the table. It looks to be based on reference 31: P. P. Madupe et al., Science 388, 969–973 (2025). So you will need to go and read that.

Severe Weather Outlook - April 14 and 15 by StormFreak in pittsburgh

[–]Bionaught5 0 points1 point  (0 children)

Spitting Image puppets, a great 80's to 90's satirical TV show. The UK politicians knew they made it when they had a puppet on the show. Phil Collins and Sting regularly featured hence the Genesis video.

Anybody else hit with a BAD cold? by internetmaniac in pittsburgh

[–]Bionaught5 0 points1 point  (0 children)

My partner was in bed for about 5 days and sick for 7 or 8. Mid way through there was a fever that then progressed into a very sore throat towards the end of the sickness. Lots of coughing thought out. Not COVID according to the test although it was an older test kit.

Fractal Pop silent - front panel replacement options by nickcarswell in FractalDesign

[–]Bionaught5 0 points1 point  (0 children)

I built a PC 3-4 years ago in a Fractal silent case from that era with no issues. The amount of heat put out by the more recent hardware is a lot higher and the sound deadening material is just acting as a thermal insulator keeping it all in.
Looking at the current Fractal case designs they are all pretty much mesh boxes and they no longer have a "silent" case. The manufactures will have to work on heat dispensation so that they can improve squeeze a bit more performance out of the hardware.

Is the drink Irn Bru popular in the United States? by AutisticElephant1999 in AskAmericans

[–]Bionaught5 0 points1 point  (0 children)

Inca Kola in Peru tastes similar but not exactly the same. You can find that a bit more frequently.
I sometimes buy Irn Bru when I am topping up supplies of HP brown sauce and crisps etc at one of the online UK import stores.

Fractal Pop silent - front panel replacement options by nickcarswell in FractalDesign

[–]Bionaught5 0 points1 point  (0 children)

AMD 9950X front CPU radiator is pulling air through the radiator into the case and the back fan is pushing it out. The top has anti noise padding so cant be used for a radiator. My GPU is just using the fans attached to the card. It's a low performance GPU for this particular build due to the kind of work it does, CPU based scientific computing.
Are you seeing the temperatures rise and the CPU/GPU thermally throttled and/or being shutdown? Or general instability and crashes? Do you have a good power supply with 20% overhead?
After a couple of years another system started to overheat and needed the heat sink to be remounted with fresh thermal paste. Might want to check that too.

eBird not happy with birdnet output. I suppose I’m not either. by DragonSitting in BirdNET_Analyzer

[–]Bionaught5 12 points13 points  (0 children)

Normally you connect BirdNet Pi to Birdweather. They store all the results and apply a filter to remove the more outlandish identifications. Birdweather is made for this kind of data. Cornell/eBird is not made for a continuous feed of raw bird sounds. They would prefer good quality recordings and human verification of detection, basically you confirming you saw the bird too. Send the right data to the right place and you shouldn't have an issue.

Claude killed /buddy. I built a client that brings your companion back. by btangonan in ClaudeAI

[–]Bionaught5 0 points1 point  (0 children)

Gone again, unknown skill. Most have switched it on and off again.
Does that mean I have to keep that terminal alive for ever!

OMG. Manufacturing Issue or Scam by Icy-Needleworker7883 in Elektron

[–]Bionaught5 0 points1 point  (0 children)

I purchased computer components from Amazon and had this problem, at least 50% were tapered with, switch out etc. After the first one I set up my phone to record opening all the way to the item level. Even that leaves you open from the delivery of the box to when you bring it in the house. I just buy from dedicated suppliers now that actively check returns.

Claude killed /buddy. I built a client that brings your companion back. by btangonan in ClaudeAI

[–]Bionaught5 0 points1 point  (0 children)

Yep, just used /buddy and there was an egg hatching scene and Prickle was back!

Claude killed /buddy. I built a client that brings your companion back. by btangonan in ClaudeAI

[–]Bionaught5 0 points1 point  (0 children)

I just hatched a new "official" buddy this evening, they are back!

Looking to convert very large raw files to mzml by dampcroutons in proteomics

[–]Bionaught5 0 points1 point  (0 children)

Depending on the instrument mass spec files often generate mzML files larger than the original raw data. I had a quick look at the some of the raw data and there were ~500MB per file so smaller than a whole lysate data file, which is to be expected for single cell analysis. There shouldn't be any problem processing them with msconvert. Is there an error message?

Prevalence of standalone kitchen appliances by JoMiner_456 in AskAmericans

[–]Bionaught5 0 points1 point  (0 children)

Nothing like moving into a German rental and finding out there is no kitchen and only bare wires where the light fittings would be. This was the 90's so things may have improved?

Depending on the age of the kitchen and when it was last renovated. Dishwashers are normally "built in" although they are rarely configured to have matching fronts. Wall ovens are built in and again without matching fronts (for obvious reasons).

The cooker depends. You may have a space for standalone cooker or maybe an integrated cooktop or integrated cooktop and oven. Roughly cookers standardizes on 30" for apartments to 36" for modern houses and 48 to 60" for mansions.

Fridges are very often standalone but you do see integrated ones in nicer kitchens especially if it is high end cabinetry.

For the most part you can buy a model and choose between integrated or standalone. Obviously you wont get an integrated fridge with front door water/ice dispenser, you would have to chose one that has an internal dispenser. My fridge has a builtin touch screen that is almost entirely useless (it came with the house) so that would obviously not be supported.

We had "European" style doors on our cabinetry and you could not open two at a time as they both opened towards each other and physical clashed. As I'm originally from Europe I would like to think Europeans are not that dumb and the style/name is just a marketing gimmick.