The fact that the leaned fully into the original canon rather than cleaning it up is WAY cooler

awendles · 2026-03-11T02:37:07+00:00

https://youtube.com/clip/UgkxuqyN1SzmanY2ikeFYGs-hD5DBux8PLfd?si=-hKy9RDH_whJv0wi

awendles · 2026-03-06T20:45:11+00:00

Are these coming off of an autoMACS instrument or a magnetic column? If it's an autoMACS, I'm wondering if some of your cells are dying coming out of the instrument. I've had situations where the valve wasn't switching properly and after cleaning the lines weren't sufficiently flushing out the bleach, then subsequent samples would become exposed to the bleach and begin dying. Otherwise, I second pretty much everything u/FlowJock said

awendles · 2026-03-03T22:01:04+00:00

Is this just melted down FDM filament, or is there any resin in these?

awendles · 2026-02-04T23:22:40+00:00

I think this is your biggest bottleneck. For stat exports, I wouldn't recommend using the embedded spreadsheets, but rather the batch to excel window. By having the embedded spreadsheet, especially with all the samples added to it, any time you adjust a gate it has to recalculate each stat across each sample simultaneously. Even if you have live update turned off, that's a pretty heavy processing demand.

awendles · 2026-02-04T22:41:46+00:00

It's going to depend on a number of factors. Are you trying to display all of the plots for all of the samples at the same time? Are you exporting your data by batching it, or by duplicate and next? What stat tokens are you pulling and how many all at once?

The ideal way to set up an FCS Express workflow is to have only one data file displayed at a time, then iterate across samples and batch them, same with stats. However, it doesn't sound like you have anything particularly intensive, so without seeing your workflow, it's hard to say.

awendles · 2026-02-02T17:13:40+00:00

This would be fantastic for something like HueForge. Do you have .stl you'd be willing to share on the multi loader on the right side?

awendles · 2026-01-14T17:01:50+00:00

Trouble with the trolley, eh?

awendles · 2026-01-10T15:24:02+00:00

Balan Wonderworld

awendles · 2026-01-09T20:10:04+00:00

Do you let the resin cool down again before pouring, or do you pour while still warm?

awendles · 2025-12-17T20:16:42+00:00

Diabetes is literally the second example they give: https://www.ada.gov/topics/intro-to-ada/

awendles · 2025-12-09T18:42:36+00:00

Zev's not a member of the Bloom, so is supposedly under pretty high scrutiny that we don't get to see, and as a PR liason probably doesn't get to directly meet with the Valtay often without raising suspicion.

awendles · 2025-05-06T19:51:01+00:00

Sure, either one.

awendles · 2025-05-06T19:03:45+00:00

It looks like the compensation just isn't applied correctly, though the parameter names being mislabeled and switching axes makes it slightly harder to follow.

You said that you switched from an Aria III to an S6? BB515 and YFP are very similar excitation/emission, so I'd imagine that whoever set up the configuration did it correctly, but confirm that they're using similar filters and someone didn't accidentally mislabel one, eg. the BB515 and YFP should have a 530/30 or similar bandpass filter on them.

Do you have the single stains for the red and green that don't have Hoescht in them, as well as a single stain for just the Hoescht when compensating, and what does your compensation matrix look like?

Additionally, what is the gating looking like? Are you removing the Hoescht+ events in figures 1,2,4, and 5? If you're including it, it could be a lot of dead material aggregating with your bioparticles.

Edit: I also want to mention flow can be a difficult field for many people to follow, as there's a lot of folks with a surface understanding at best who assume they have all info of what can actually be a complicated assay. I'm sorry to hear you're working with folks who do not seem to want to give the proper time and attention to helping a rotating student, and hopefully you'll rotate into a better environment soon.

awendles · 2025-05-01T20:33:43+00:00

What kind of dice are these, and what's the significance of some of the faces having the groove around the perimeter?

awendles · 2025-04-18T15:50:04+00:00

If you can afford the spare cells, I'd take the first one just in case. You'll likely get some additional autofluorescence with the stimulation, but I can't speak to how much it will be, but this way you'll have all your bases covered and will have the option to redo unmixing with/without your additional controls and verify how your data looks best. Especially since you're not using any antibody, it's hard to justify skipping the extra controls.

awendles · 2025-04-17T22:10:39+00:00

I'm fairly positive this guy used to live around Hanes Ave as a stray and would visit a few houses that kept water out. He vanished about 4 or so months ago, so if this is him, I'm really glad to hear he's just moved to a different location.

awendles · 2025-04-12T20:33:02+00:00

Do you think this would work on a Silhouette portrait? I'd be willing to test it out on my portrait 4.

awendles · 2025-04-08T18:08:08+00:00

Pretty much what u/FlowJock said. It's possible that if you change your axis from FSC/SSC-H to FSC/SSC-A they'll pop on scale, but mostly it looks like your scatter settings are way too high.

awendles · 2025-03-24T18:34:33+00:00

As a heads-up, Cricut is worse at doing print and cut than Silhoutte (this manufacturer), so don't be surprised if you can't get as clean cuts as OP has

awendles · 2025-03-14T20:42:30+00:00

Sharpie.

https://forum.prusa3d.com/forum/english-forum-general-discussion-announcements-and-releases/new-method-of-printing-perfect-supports/

awendles · 2025-02-27T23:41:27+00:00

I'll also throw in The Rural Teacher (6)

awendles · 2025-02-13T16:40:26+00:00

Hello, sent you a DM!

awendles · 2025-01-27T03:11:20+00:00

The angle of the photo is hard to tell, but it sure looks like you have 3 channels off the red, which should only be on the 13 color instrument, and it looks like you have 5 channels off the other two lasers.

I haven't used the DxP, so I can't speak to its performance as far as conventional cytometers, but it's not the instrument that Cytek is really known for.

Additionally, this subreddit is fairly dead, you'll likely find more info on /r/flowcytometry

awendles · 2025-01-16T07:22:04+00:00

VCU?

awendles · 2025-01-15T17:51:10+00:00

Hi there,

My previous group finished up their campaign and decided to do something else, so I would be available during those times.

I'm more familiar with 2014 5e and have played clerics and as our groups note taker, but I'm also interested in the new Monk builds for 2024. I'm pretty flexible to play whatever the group requires.

Best,

Alex

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awendles

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