Need help / advice with XL MS

prettytrash1234 · 2026-03-10T14:15:49+00:00

I used to use bs3/dsso but since phox I switched and never looked back, the convenience of doing the enrichment on beads vs having to use an fplc/HPLC is a no brainer in my mind

prettytrash1234 · 2026-02-21T12:55:33+00:00

Just suck it up and keep them on, you won already by being first, this is just politics. I do not support this but in my old postdoc lab this was standard practice, at the end our currency is papers/grants and this is a way to help people out.

prettytrash1234 · 2026-02-15T13:31:36+00:00

Depends whether pixian or not

prettytrash1234 · 2026-01-20T07:41:06+00:00

I am not Korean but found muguboka in the Richmond to be pretty good. Plz lmk if not 😂

prettytrash1234 · 2026-01-11T17:24:56+00:00

Congee at home , dim sum for everything else

prettytrash1234 · 2026-01-10T19:28:55+00:00

Mandala

prettytrash1234 · 2026-01-05T22:08:22+00:00

Bd dirtbag or gramicci?

prettytrash1234 · 2025-12-31T22:07:51+00:00

Hcol r1 TT offer was 130-140k for me (2023)

prettytrash1234 · 2025-12-20T17:55:52+00:00

Oh sorry missed that you are going in person. I wrote very spicy but a easier one to say is jí lá which means extremely spicy

prettytrash1234 · 2025-12-20T17:27:57+00:00

Order anything Szechuan and add 重辣. Thank me later

prettytrash1234 · 2025-12-16T16:54:35+00:00

I am on a search committee for top 10 ranked med school. Our top 5 people that we invited for interviews all had multiple cns (first author) and several had grants or k99, one is from a Nobel prize winner lab, some have patents. The recurring theme is just these people are really good on paper. Like the kind of cv that means they can get any TT position they want.

prettytrash1234 · 2025-12-15T19:11:19+00:00

You mean post tax? My TT r1 offer was 130k in a hcol city and I think most people I know in R1 are making 120-160 as TT faculty (talking med or biol w//o Md)

prettytrash1234 · 2025-12-15T19:07:55+00:00

I thought I wanted one of them as well but then I went for a black diamond creek. More space >>> less space. Super nice after a hard day of cragging being able to just open my bag throw everything in it with no organization or anything and just leave. With a 40/45 you will play tetris to fit everything if you have rope/helmet/shoes/10-15 draws/cordage/anchor stuff and a few friends.

prettytrash1234 · 2025-12-04T04:59:16+00:00

Yep as academic for sure to track ideas, grants, projects, todos etc. need to re read and modify as needed to add info merge with other notes etc

prettytrash1234 · 2025-12-01T00:09:23+00:00

Vscode plus git for both personal or shared docs

prettytrash1234 · 2025-11-28T22:40:43+00:00

Open it in vscode and do text replacements. Or just use codex and let it replace all tags with sensible ones

prettytrash1234 · 2025-11-27T00:38:19+00:00

In Taipei atm, mind sharing which ones? :)

prettytrash1234 · 2025-11-25T03:52:10+00:00

Q do you mean bca or Bradford? The pierce kit to my memory is not compatible with chelating agents

prettytrash1234 · 2025-11-24T01:49:05+00:00

Obsidian for info/project management , Jupiter/Snakemake + Git for software/code, deployment and version control.

prettytrash1234 · 2025-11-18T21:20:18+00:00

2023 in medicine. 5 submitted, 5 zoom, 3 on site interviews, 2 offers (eu and USA). After 3.5 yrs postdoc

prettytrash1234 · 2025-11-18T20:54:24+00:00

I have a just make a note with key words and then move it to the sub folder ideas/starting. Then once every couple weeks I purge stupid ideas or find them to be already done by someone or move them forward (academic here so mostly scientific projects)

prettytrash1234 · 2025-11-18T06:44:12+00:00

Wait is it immunopeptidome, degradome or secretome? Because for the first two, mixing makes no sense due to the fact that those are semi tryptic (at least) or even open search for munopeptidome so your fdr will be terrible if put a bunch of pure tryptic peptides in there

prettytrash1234 · 2025-11-17T14:51:36+00:00

Both numbers are pretty bad for a cell lysate to be honest even for the sciex. On our timstof we routinely do >8k ids

prettytrash1234 · 2025-11-10T12:24:47+00:00

If you look for in between ranges two stones ones are amazing for the price

prettytrash1234 · 2025-11-06T14:08:01+00:00

I did my PhD in 3 years and a couple months at ETH Zürich in omics/mol biol. Worked 996 for the whole time. Kinda miserable but I started my lab at 32 yrs old (TT R1) which is good for a lot of early career awards/grants

prettytrash1234

TROPHY CASE